Video of Assembly (shows assembly of trench-down device, note chip is placed trench-up on fixture A1)
Trench-Up vs Trench-Down
The orientation of the chip in your device will depend on your application. For example, trench-down devices are useful if you desire a completely flat monolayer of cells and want to avoid changes in geometry. Trench-up devices are crucial for those using high resolution imaging and need to minimize working distance of cells and the objective.
When assembling your device, you place the chip in the OPPOSITE orientation than the desired final orientation, as shown in the video.