Two weeks ago (as of the time of writing this post), I wrote a post on a dead end filtration experiment that I ran in order to demonstrate particle capture in pores, modeling a simple, purified exosome capture system that…

This is the second in my series of posts that will be on my model exosome system. As I am trying to define the appropriate conditions for separating exosomes from blood and other fluids, I am first going through and…

In trying to determine the best conditions for capturing exosomes, one of the important questions that we have is what produces the case where exosomes are caught directly in the pores, not on top, but flush with the surface. This…

A few weeks ago, Josh and JP brought me some 3 window microslit membranes that had edge aligned 8 and 9 μm slits. While these may seem to be quite large for most applications, they are in fact perfect for working…

This is my third post that is related to the NanoAuger system that I had the opportunity to use while I was home over break. However, instead of quantum dots and exosomes, this time I will be talking about data…

Alright, so this is kind of a big update and I will probably split it into a couple of parts. Basically, in a search for a way of confirming that the particles we are seeing on the membranes, I have…

Since I am posting data that is acquired using Auger electron spectroscopy, I thought that it might be a good idea to write a post about the theory of the instrument, to better understand why this data is useful and…

In attempting to generate control systems for our exosome work as well as to potentially develop a system that would help some collaborators at the University of Illinois, I have been working with the ultracentrifuge again to hopefully isolate a…

This is pretty much an update to a previous post on trying to identify exosomes using an immunogold staining procedure, FIB lift-out and the TEM. After multiple attempts to cut out a section of the membrane for attachment to a…

As a step to identifying the particles that I am capturing in my systems, I am attempting to do an immunogold labeling with a CD 63 primary antibody and a 10 nm gold conjugated secondary. It is possible to run…