Author: Andrea

Co-Culture of ADSCs and HUVECs

Scope: Adipose derived stem cells were seeded onto  Corning 3um tracketch membranes, human endothelial derived stem cells were seeded onto the membranes approximately five hours later. Numerous control wells were also seeded with ADSCs both on membranes and on the

Posted in NRG

Co-Culture of ADSCs/HUVECs on 0.4um Translucent Membranes

Scope: ADSCs were cultured on the bottom of the Corning 0.4um translucent membranes in DMEM+ 10% FBS+ 1% Pen-Strep. After 6 hours, the membranes were flipped over and placed into a 24 well plate. Of the four membranes with ADSCs

Posted in NRG

ADSC differentiation and networking in various environments

Scope: The purpose of this experiment was to determine which conditions are best for differentiating adipose cells into endothelial cells  and formation of networks. ADSCs were used for this experiment, variables included media and matrices. Geltrex and collagen were used

Posted in NRG
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