Yuchen and I have been tasked with integrating TEER electrodes into the Cytovu format. The general idea was to fabricate the TEER electrodes similarly to what I have done already, then add them to the current Cytovu device, using UV…
Author: Greg Madejski
Greg is a McGrath Lab alumnus (2010-2020) who incorporates nanomembranes into a variety of devices. Currently, he works on detecting microplastics as the CEO of Parverio inc.
BBB Device 2.7 Fabrication with Mortar Bonding
Fabricate ITO Electrodes Deposit 2500 Angstroms, ITO, Room Temperature Using Kurt Lesker Sputterer (Use ITO Variable Recipe, takes about 1.5hr) Tape in Microscope Slides and Coverglass into Machined Shadow Mask using Kapton Tape Anneal in Toaster Oven, 450 Fahrenheit, 1hr…
Posted in Protocols
Lung on a Chip
Links and Papers: Article with video Lung on a Chip Device Paper Lung on a Chip Application (Pulmonary Edema) Chris came across the publicity video last week and Jim asked me to look into the specifics. It’s a pretty nifty…
Posted in NRG
Biomed Microdevices (Griep et al, 2012) Summary
BBB On Chip (Griep et al 2012) Jascha sent me an article about a BBB device. Their device follows a very similar structure to that of the Booth and Kim paper, but closer to the optimized design that I created,…
Posted in NRG
Electrochemical Testing of Ultraporous Membranes as Separators in Mild Aqueous Supercapacitors
I’ve been thinking a lot about energy storage lately, and I think I’ve found a potential use for pnc-Si in Supercapacitors. What is a supercapacitor? As the name suggests, it’s a double-layer capacitor (EDLC) with very high capacitance values (1-500+…
Posted in NRG
Shadow Mask ITO Deposition Characterization
Josh has been kind enough to fabricate a shadow mask for me to create interdigitated electrodes without the hassle of photolithography and etching. In order to be able to use this method, I want to make sure the produced electrodes…
Posted in NRG
BBB Device V2.6 Flow and TEER Test
Following my fabrication process from my previous post, here are the results from my flow test of BBB device v2.6: The difference in the quality of the electrodes is apparent from these images. The microscope slide electrode was difficult to…
Posted in NRG
BBB Device V2.6
Given the results of my previous post, I am reworking my design to make it more manufacturable. BBB Device V2-6 Compressed The major change compared to my last design is the inclusion of a full length microscope slide and…
Posted in NRG
Autoclaved BBB Device V2.5
To prepare the BBB devices for cell culture, they must be sterilized. To that end, I wanted to see if there were any adverse effects from autoclaving the device. Recipe 4 on the U of R autoclave is a dry…
Posted in NRG
TEER Testing BBB Device v2.4 with Shrink Wrap and Media
After some changes to my fabrication process, I can report a qualified successful TEER test with media (HBSS) in both channels. I utilized a shrink wrap method of fabrication as seen in a previous post. The test was not perfect…
Posted in NRG
