This is kind of a shot in the dark, but I wanted to see if I could get a zeta potential for pnc-Si that was as high as predicted by the electroosmosis results. First I tried to get the membranes…

This is something that’s been living on my knowledge page, but I think it’s a good idea to put it up here to get some discussion on it. Probstein gives the following equation for electroosmotic velocity in his book Physicochemical…

A few of the newest SC wafers came back with intact samples without any pinholes. First here is a list of the experiments that we want to do in our lab with intact/pinhole free membranes 1. Protein separations – Requires…

Even though we don’t have defect free membranes, I wanted to tweak the gold setup using membranes with pinholes. Previously I was using the small scale diffusion chamber that I use for protein separations. This setup has a retentate of…

First off – OK now on to the science. Wafer 747 has a huge amount of pinholes, and in this study I wanted to see how these pinholes would effect electroosmosis.  I will show results here from two experiments with…

In the previous post, I used a compound error bar that took into account both image and operator variability. While that seemed to make things a little better, we were still having a problem with w705. I reprocessed a couple…

I wanted to attempt pressurized and diffusion separations of brain extract using the new movable wafers. I tried pressure cell separations of 1:20 BBEC (clarified brain extract) at 2psi for 40 min. The flow rates were better than the standards…

Previous Post The previous w670 pressurized flow gels showed one trial with a lower cutoff than w673 and one trial with nothing passing through.  This experiment was run for 40 min and in each case I saw only 0-3 uL…

The movable cutoff gels were more or less successful, but were too dirty for publication (see knowledge page). I’ve been trying to rerun them so that they look nicer, but the darker stain that I used has brought out a…