Today I built a small scale electrophoretic cell using the components from Lingyun’s study.  I drilled out his cell and affixed a coverslip to the bottom.  I set up a pinholed membrane just like our standard diffusion assay (with silica…

I oxidized 2 intact chips from w639 using the following protocol: 10min with secondary generator, 15min with both primary and secondary generators.  This protocol is slightly different than my normal protocol (10min 2nd, 5min 1st+2nd, 10min 2nd) because I messed…

I’ve tried to get some more data for the phenomenum that may be electro-osmosis in our electrophoretic cell.  In the last post I looked at the rate of passage of 1x PBS in pinhole membranes from w621.  After that I…

JP handed me a stack of wafers today, and I went over to the Krauss Lab and with the help of Jack Calcines got some AFM images.  I didn’t have much time to play with, so I tried to get…

Electro-osmosis: Yesterday I tried to study the electro-osmosis-like phenomenon that we were observing with the electrophoretic cell.   All membranes again had pinholes.  I set up the wells as before, but I would remove 50ul from the well which the negative…

We were worried that Friday’s experiments were giving us a false positive results. If the electrophoresis breaks the membrane, then we’d expect to see the rhodamine rush through just like it was in our experiments. It’s too hard to view…

Because of Harold Smith’s project, Rachel and I have been working on various methods to get proteins through a pnc-Si membrane and onto a polymer membrane.  The particular system that Dr. Smith wants to study is very sensitive to ambient…

One question we recently had was what does it look like when we perform a separation experiment when a membrane has a pinhole. I selected two membranes off 621 that appeared to have pinholes on both slits. I applied 1:2…

Bill Bernhard’s group approached us about using pnc-Si membranes to separate DNA species by diffusion.  Bill’s lab studies the effects of ionizing radiation (ex. X-rays, gamma rays, UV light) on DNA using electron paramagnetic resonance (EPR).  There are three major…

This was something I set up a few days ago and finally got a chance to run the gel.  I was curious to try this after the previous forced separations using brain extract.  If you recall, the “separation” using the…