In this experiment, I soaked membranes (from either wafer 1064 [150W deposition power] or wafer 1065 [100W deposition power]) in different fluids that are standard use in cell culture applications, or which provide a similar situation as could be found…

In evaluating the usefulness of our membranes for cell culture, it is important to understand if and how cell growth is affected by the surface.  The desire is that they be consistent with standard culture substrates (i.e. Tissue Culture Plastic…

In a previous post I had shown the relative fluorescence of various substrates as normalized to glass.  1um beads (either green or red) were placed onto each surface and imaged with constant EM Gain, exposure time, and intensity.  Bar graphs were…

In this previous post (also some in this post), I had displayed the difference between the 120nm SiO2 microporous membranes and four different iterations of Track-Etched insert membranes in reference to nuclear (DAPI, blue) and actin (Phalloidin, green) stain. I recently…

While performing an experiment to study HUVEC growth rates on various substrates, I observed an interesting phenomena on the oxide membranes.  I was using 120nm, nonporous, 600C annealed SiO2 membranes in this trial.  The cells were observed at least once…

Method: Oxide membranes in some conditions have been shown to wrinkle over time.  To study this, three different varieties of SiO2 membranes were observed (1000C nonporous 120nm SiO2, 150W deposited microporous low-density 300nm SiO2, and 100W deposited microporous low-density300nm  SiO2).…

After seeing in previous posts how optically transparent the SiO2 membranes are, I went about seeing if I could quantify this in comparison to the SiN membranes and also CoStar PET track-etched membranes. In order to do so, I ‘seeded’…

I’ve run a few comparative studies with the CytoVu devices that have SiO2 membranes in them.  From a previous NRG discussion, here is the comparison of a single location with only the fluorescent images, and that same location but with phase included:…

In this trial, bEnd.3 cells were seeded onto three single-well CytoVu devices with SiO2 membranes coated with a 1% Geltrex coating.  After ~27 hours, the cells were fixed and permeabilized (the fixing may be only partial though, as the solution…

After experimenting with some different coatings and membrane types in single-well CytoVu devices, here are some representative images of the results: SiN (Nitride) membrane with a Geltrex non-gel coating @ 44hr: 10x: 20x: 40x: SiO2 (Oxide) membrane with a Geltrex…