Lately I’ve been working on building computational models which can be used to make predictions about the behavior of a diffusion experiment, to allow for the collection of large pools of data without the difficulty and time consumption associated with…
Category: NRG
A New Platform for Pressure-Driven Voltage-Gated Separations
I’ve been trying for some time to make chips that can dynamically change their surface charge. To this end I’ve made the following NFT chips, which have 12 nm of silver coated in 10 nm alumina: Note the Aluminum…
Posted in NRG
Surface-Charge-Dependent EO Pumping Behavior Persists (weakly) at Low Applied Voltages
In a previous post (link) I presented a video that showed that the voltage applied to a silver film changed the pumping behavior of a membrane. The key data is summarized by the following powerpoint slide: My most recent post (link –…
Posted in NRG
Modeling pore clogging
In recent posts we have tested the total volume of filtrate as a function of time in the centrifuge. We used NPN with different average pore sizes and we studied 100 nm and 20 nm NPs as well as IgG.…
Posted in NRG
Electrical Characteristics of Silver/Alumina NFTs
Just before BMES I was finally able to electrically characterize my NFT chips. Previously, I had been trying to collected IV sweeps using the following circuit: The problem with this was that, as Jess discovered, the membranes have almost no…
Posted in NRG
HUVEC-ADSC Co-Culturing on Membranes
In response to last meeting: We tested more controls to help support our idea that the ADSC are migrating toward HUVEC network and prematurely breaking them down. We used a 100% Geltrex coated cytovu gasket to test if ADSC are…
Posted in NRG
BMES 2014 Talk Comments – Greg
Here are my thoughts on the talks I attended at BMES: OP-Thurs-1-11. BioMEMS I Oral Thursday, October 23, 2014 8:00 AM – 9:30 AM Room 008A Moderator 1: James Tunnell Moderator 2: Catherine Klapperich 8:15 AM – 8:45 AM High-throughput…
Posted in NRG
First workable clams
After a lot of trial and error with the hinge, I was able to create something usable!!! Apparently I cannot upload the .stl file or .skp file to the blog directly for “Security Reasons” I printed this one out of…
Posted in NRG
Optimization of HUVEC Network Formation in Geltrex On and Off Membranes
Our motivation was to look into how ADSC influence HUVEC network formation. My first objective was to investigate the optimal cell count, the best volume of Geltrex and if it was better to use M200 media with or without growth…
Posted in NRG
Makerbot Replicator 2x Open for ABS prints
Recently, Jim has purchased a Makerbot 2x printer for our group. I’ve spent the last few weeks printing and debugging. It has the capability to print many different types of material, along with 2 separate nozzles. I have been able…
Posted in NRG
