We borrowed a method first published by Jahn et alii about 7 years ago, in which lipid dissolved in ethanol are streamed through a central channel and compressed by 2 streams of  buffer (we used PBS).  This hydrodynamic focusing results…

In this post I address every objective we started with and summarize our results: a) Building of research capacity at Fisher. —–> We would like to to produce our lipid-based nano particles in a cheaper and more reproducible way. For the…

Overview: After trying an initial experiment of growing ADSCs on TCP and 3um translucent (TE) membranes and then placing them onto TCP or 3lp SiO2 membranes, it was determined that further studies should be conducted. For this experiment we grew…

Overview: Bob etched 4 non-porous membranes, on the backside of the membrane. HUVECs were to be grown on these etched non-porous SiO2 membranes and compared to unetched non-porous membranes. Below Bob describes how the membranes were etched: “Four of the non-porous SiO2…

Overview: The study of ADSC spreading on various surfaces was conducted to determine if porous surfaces and the porosity of the surfaces had any effect on cell spreading. Eight different surfaces were studied and each of the membranes were SiO2,…

As part of the ongoing study of NPN membrane filtration characteristics I used the SEM to look at membranes after they were used.  Unless otherwise indicated, files with a “t” (top) indicate the chip was membrane side up.  “b” stands…

UPDATE: Some data presented here is out of date. For up-to-date data, see my next post here. — Lately I’ve been performing comparison experiments between our NPN membranes (wafer 1132, 3 slot SEPCON, 40-48nm pores and 13-20% porosity) and commercial…

Last week, I worked with Kevin to create a prototype cell culture device that could be compatible with his Raman system in Nottingham. The space constraints of the physical system are challenging: The overall working distance of the system must…

In this post, I’m going to discuss the results of the different PEG molecular on silane samples. We used same condition to make 4 chips for each sample. And we treat them with BSA, IgG, and PBS to rest the adsorption. We…

The following plot is an attempt to summarize recent work measuring rate of flow through a membrane as a function of concentration.  We investigated 100 nm NPs, (totally blocked), 20 nm NPs (partially blocked) and IgG (mostly passed).  In all cases,…