Author: Alec
Alec joined the McGrath Lab as a pre-doctoral student in 2017. His focus is on designing and implementing microfluidic devices for studying sepsis associated vascular damage. His work includes membrane fabrication, human cell culture, and advanced device fabrication in collaboration with Aline Inc.

Confocal Analysis of Apical and Basal ICAM-1 Expression Following Polarized Cytokine Delivery

Posted in NRG

Microvascular Mimetics for the Study of Leukocyte-Endothelial Interactions

Here are the figures for Tejas’ paper that is in the final stage of drafting:

Posted in NRG

Polarized TNF-α Update: rhIL-8 and PMN Persistence

Introduction In a previous post I introduced the concept of endothelium apicobasal polarity and the potential to utilize this concept for future sepsis drug development: Endothelium Apicobasal Polarity Regulates the Innate Immune Response In Vitro [In Progress] In this post,

Posted in NRG

Etaluma™ Microscopy: A Fully Incubate Microscope for Live-Cell Analysis and PMN Migration Videos

Introduction Etaluma is a company that builds high resolution microscopes in a compact format for integration into cell culture incubators. This shifts from the traditional stage top or incubator cage approach to live cell imaging to allow for steady time-lapse

Posted in NRG

Endothelium Apicobasal Polarity Regulates the Innate Immune Response In Vitro [In Progress]

Introduction Currently, no FDA approved drug exist for the treatment of sepsis. Past sepsis pharmaceuticals attempted to target mediators of the innate immune response. These drugs, however, struggled to balance remedial immune suppression from complete immune knockdown in a patient

Posted in NRG

Alec’s Proposal Exam Documents

Here are my proposal exam materials for reference. Proposal Document: Proposal_Salminen Proposal Presentation (No Animations or Videos): Proposal Presentation_No Animations

Posted in NRG

Microfluidic Permeability Assay for In Situ Monitoring of Endothelial Barrier Properties

Introduction As part of my thesis, I have been working on developing an assay for measuring vascular barrier properties within our microfluidic devices. Traditionally, small molecule permeability measurements are performed on cells plated in a Transwell cup. The basic protocol

Posted in memBrains, NRG

Updates on Dual-Scale Membranes (µNPN): Collagen Gel Backing and Shear Validation

Introduction In response to recent requests by our reviewers, I am looking to address two main concerns: 1) How does a collagen gel backing affect leukocyte transmigration, and 2) How does shear influence leukocyte transmigration. While we believe these areas

Posted in NRG

BMES 2018 Takeaways

BMES – Atlanta 10/17/18-10/20/18 F-actin Alignment Quantification Using HSB Color Coded Maps ImageJ Plugin: OrientationJ Purpose: Quantification of F-actin alignment using ICC. HSB map provides a visual representation of fiber alignment with color codes related to hue:local orientation, saturation:coherency, brightness:fiber

Posted in NRG

Effects of Luminal and Abluminal TNF-α on Neutrophil Migration

Introduction In severe sepsis, a primary infection gives way to a maladaptive immune response leading to wide spread edema and multiple organ dysfunction. The initial infection can occur in many peripheral tissues throughout the body. The pathogen associated molecular patterns

Posted in NRG
0
    0
    Your Cart
    Your cart is emptyReturn to Shop