In my last post, I presented the results of my BBB TEER experiments.  For the BBB co-culture, there was a spike in TEER.  I hypothesized that this was caused by media acidification by rapidly proliferating glial cells, which in turn…

This post presents the data from all of my TEER experiments of BBB co-cultures, with bEnd3 endothelial cells on the bottom and NG101815 glia on the top of PET and pnc-Si transwells.  For all experiments, I used 50000 cells/cm2 as…

A few weeks ago, I posted some high resolution (100X) images of BBB co-cultures stained with 3 different colors.  I also included the z-height of the different focal planes of cells on either side of PET transwells.  In this post,…

To follow up my last post on air-blood barrier TEER results, I wanted to show some Live/Dead images of these samples.  These are stained after the 14 day experiment. In the following image, you can see normal bEnd morphology on…

I finished 3 independent experiments of air-blood barrier co-cultures – these consist of bEnd3 endothelial cells on 1 side and RLE-6TN lung epithelial cells on the opposite side of the membranes.  We didn’t expect these cell types to potentiate TEER…

Hi everyone! I’m sifting through the mass of data I collected during my last month and am compiling posts to update everyone. This post shows the latest with the microfluidic chamber that Henry and I designed.  In my first post…

Over the last couple of weeks, I’ve been doing confocal fluorescence microscopy over at the Med Ctr.  I was hoping this tool would clean up some of the background fluorescence in my immunofluorescent images.  More importantly, the confocal can take…

As a proof-of-principle, Henry and I have been working on a microfluidic device for flowing blood cells/particles across cultured cells on pnc-Si.  More on why this is important later.  We’ve come up with a prototype and I’ve been testing it…

One of the points I’d like to discuss in my co-culture paper is the advantageous imaging qualities of pnc-Si compared to ‘translucent’ commercial transwell membranes.  This has been evident in many of my posts of Live/Dead stained cells, even at…

I’ve been trying to get multi-color images of endothelial cells and glial cells on transwell membranes in order to compare image quality on glass, pnc-Si and polymer membranes.  Specifically, I’ve tried staining endothelial cells green with VEGF mAbs and glial…