I’ve repeatedly seen cytotoxic edge effects when I grow bEnd3 cells on the bottom of PET membrane transwells.  Here’s a post of images.  I’m getting closer to co-cultures of bEnd3 and NG108-15 cells on opposite sides of membranes and I’ve…

I was motivated to scan our membrane edges after looking at this image from the paper Maryna presented last week: I found a couple of pnc-Si samples with membranes still attached to the edges.  I also found one sample with…

This is my first attempt to grow astrocytes (NG108-15 cell line) on PET and pnc-Si transwells.  These are passage 8, seeded at 50000 cells/cm2 and grown for 7 days.  Of the 8 pnc-Si transwells (RTP’ed SC104) I made, all but…

In my previous post, I showed images of bEnd (P8) cells on PET and pnc-Si transwells.  These were seeded at 105 cells/cm2 and grown for 14 days.  This post contains the TEER data.  I simply normalized all values to the…

I continued to run some of the samples that I described in this post last week.  These are P8 bEnd3 cells, seeded at 100000 cells/cm2 (just below confluence) and grown for 14 DAYS.  The pnc-Si was SC126 that was RTP’ed. …

With the previous 2 experiments, I made a couple of 6-slit pnc-Si transwells.  These chips were from the cell culture wafers I had made a while ago.  These samples (SC126) were RTP’ed before use. My improved technique, combined with the…

Along with the PET data I just posted, I also examined cells on pnc-Si transwells.  The following images were taken at 20X (through the bottom of a 24-well plate), and the cells are passage 8 bEnd3.  They were seeded on…

A while ago, I posted some images of cells grown on commercial polyester (PET) transwells.  Here’s the post.  I found that there was an annulus of dead cells around the edge of the transwell when cells were grown on the…

I took some force curves a while ago to see if there was any difference between wrinkled and taut membranes.  Here, I present only the data with a 15nm ‘trigger’.  The trigger is a user-defined parameter that sets the maximum…

I’ve been thinking of demos/experiments to do with my upcoming class and one of them is SEM.  I was thinking of handing out some unknown nanoparticle solutions and have them figure out the size and maybe material by SEM, AFM…