Introduction Motivation: We want to assess if the bladder cancer cell line T24 would influence the baseline characteristics of bladder epithelial cells (BdEC, a.k.a. ATCC® PCS-420-010™) when the two cell types are cultured together. Ultimately, we are interested if the T24…

Introduction Motivation: We want to assess if the bladder cancer cell line T24 would influence the baseline characteristics of bladder epithelial cells (BdEC, a.k.a. ATCC® PCS-420-010™) when the two cell types are cultured together. Ultimately, we are interested if the T24…

Hi all, recently we improved cell tracking with the use of SiR-DNA label (see highlights below).   random migration:  A. Representative cell tracking from three independent migration experiments, color-coded by the color bars to the left in red, green, and…

Traditional Wound Healing Analysis (the implicit gold standard) Wound recovery is reported base on the closing of the two wound edges.   Proposed Wound Healing Analysis (cell counting/tracking methods): Wound recovery is reported base on the migration/movement of the wound…

Intro/Motivation:   SiR-Hoechst is a far-red DNA stain for live-cell micro/nanoscopy and has been shown to be non-cytotoxic. I mentioned to Tom that if we can label the cells we can better automate the cell tracking for faster analysis. Tom knew of this…

Intro Recently we have been collaborating with the Moghaddam lab from the University of Florida, which specializes in the fabrication of graphene oxide (GO) membrane, to study the use GO membrane as a toxin filter or absorbent in hemodialysis and live support…

[NOTES] BMES 2017 highhlights

Hi all, This is a “take two” of the mock figure for our manuscript titled “Porous substrates influence early endothelial migration and the associated fibronectin fibrillogenesis”. Many new figures are produced based on the reviewer suggested experiments. It is likely…

Hi all, Recently we finished drafting the manuscript titled “Porous substrates promote early endothelial migration at the expense of fibronectin fibrillogenesis.” This manuscript will feature the cover illustrated by Brad (our new medical illustrator in the Gaborski Lab):   Long…

[presentation] HUVEC migration and fibronectin