We are working with the Shestopalov group to silanize the nanoporous silicon nitride (NPN) membrane chip. In an ideal scenario, the silane would then be conjugated to PEG to ward off protein adsorption. In the presented work, we study protein adsorption…
Author: Henry
SEM of geltrex coating on SiN membrane
Hi all, A while back we have shown that geltrex coating enhances the attachment of HUVEC on SiN membrane . Jim believes that geltrex may be extended an “all-purpose” coating to promote the adhesion of different cell types to the SiN…
Posted in NRG
HUVEC alignment on SiN membrane under flow
Hi all, A while back I have posted about my attempt to culture bEnd3 (mouse brain endothelial cell) underflow, which failed to maintain bEnd3 proliferation and did not promote alignment under flow. As an alternative/control, I switch the cell types…
Posted in NRG
bEnd3 culture under flow for BBB mimetic (sort of)
Hi all, For the past one month I have been working on a height-based device for culturing bEnd3 (mouse brain endothelial cells) under flow. Ideally, this device is to constitute a blood brain barrier (BBB) mimetic. Device Full View…
Posted in NRG
bEnd3 culture for BBB mimetic (open static system)
Hi all, Currently I am working on a cell culture system to mimic the blood-brain-barrier (BBB). Essentially, growing endothelial cells (bEnd3) on one side of the membrane, and pericyte and/or astrocyte on the other. Below is the layout of the…
Posted in NRG
Error made when assuming identitcal pore size during simple flow modeling
Hi all, Sometimes for flow modeling I assume all pores to be identical in size. However, since larger pores allow much higher passage of flow, the above assumption will generally underestimate the prediction of flow. Presented here is a…
Posted in NRG
Sneak Peak of a Simple “All Purpose” SiN System
Hi all, Presented here is a prototype of an “all purpose” SiN chip system for use in cell studies. The reason it was called “all purpose” because it is intended for simple cell culture and migration/chemotaxis studies. This prototype is…
Posted in NRG
simple alignment tool for microfluidic assembly
Hi all, This post showcase a simple (low-tech) alignment tool for assembling the different components of a microfluidic chamber. Essentially, the alignment pieces are made from two index papers cut using the Silhouette Cameo. The two cut index papers are…
Articles for (Jun/06/2014)
Hi all, i have to present for a biomechanics journal club meet next Friday (Jun/06/2014) and figured that i might as well double-dip the presentation. Currently i am leaning forward to present this article: Takano (Futai) microfld nanofld 2012…
Posted in NRG
Protein Adsorption (are we saturating the substrate?)
Hi all, Today I would like to briefly mention the important parameters to consider when saturating a substrate with proteins by simple adsorption. More specifically, the concentration and time required to fully saturate a substrate ares estimated for systems with different…
Posted in Knowledge, Knowledge (Public)
