5nm BSA-Au appears to be 12.7nm.10nm BSA-Au is 18nm. Dilution dependent size change of 5nm BSA-Au. One theory for the size change was that residual BSA was combining with the Au peak and for some reason dilution of the BSA…

w312 Number of Pores HistogramContributed Area Histogram 5nm BSA-Au is detected in the filtrate after 24 hours. 10nm BSA-Au is not. The 5nm registers around 10nm after conjugation and the 10nm is closer to 15nm. This selected membrane is not…

Directory containing article and presentation Nature Article about Willem Kolff

20nm polystrene beads in 1x PBS passed through w218. This wafer looks a little strange, like it was not fully etched (Dave do you know what happened to this one?). This was just a first stab at the benchtop diffusion…

The new smaller EQ setup that Tom came up with were tested in these experiments. The benefit is that there is much less dilution of our filtrates. 500ul of our sample (in these cases we tested 1.0 mg/mL and 0.5…

First Try is detailed in this post. Three membranes from w334 were used to separate depleted serum. As before we see that all of the proteins pass through the membrane, and again we can observe the one middle band that…

This gel shows the separation using two 100k Microcon membranes and 1mg/mL brain extract. 500uL of 1mg/mL brain extract was loaded into the Microcon and spun at 14000g for 5min. 400uL passed through the membrane (100ul remained on top). It…

This is a side view schematic of the experiments I’m setting up in Mike and Derek’s stir cell. The membrane separates two wells. In the well where the membrane is flush with the wafer I add 300uL 5nm gold in…

I am trying to build a helpful spreadsheet that outlines all the experiments perfomed with a particular wafer. It also lists the conditions and quality of the wafer. Any other suggestions would be helpful. To view the spreadsheet, click here.

I’m currently trying to compare separations between different complex protein mixture. This was my first crack at depleted human serum. This mixture has been processed so that the most common blood proteins (such as albumin and immunoglobulins) have been removed.…