Author: Jessica Snyder

A closer look at separations

UPDATED 12/12/07 – Added data on albumin. In these gels, the band from beta-galactosidase appears to overlap with phosphorylase b making it difficult to determine which protein passes and which is retained. I have used Mike Bindschadler’s pore imaging software

Posted in NRG

CB Report Update

Today I looked at 3 more wafers: w158 (9/7/07) – some pinholes, overwhelming amount of very fine debris, not cbs as far as I can tellw160 (9/7/07) – some pinholes, debris, no cbsw171 (9/26/07) – many pinholes, lots of debris,

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New Diffusion-Based Separations

Two simple diffusion separations were performed before break using membranes from wafers 205 and 213 (locations unknown as these were salvaged/misplaced chips). I ran the samples I collected on a 10% SDS-PAGE gel today. It seems that the membranes were

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Update to the Cigarette Burn Report

I stripped and viewed samples from wafers 186, 185, 184 and 182. None of these samples had cigarette burns. Follow this link to the updated summary: cb_report Previously I had noticed cigarette burns (small ragged pinholes) on samples from wafers

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11/10/07 Albumin/Cytochrome C EQ Tests

This is a 12% SDS-PAGE gel of the human albumin (alb) and cytochrome c (cyt c) EQ tests. Both alb and cyt c passed through the membrane, while the cyt c impurity (middle band) and alb impurities (high bands) did

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