Previously, I had data showing that 5nm gold in 10mM salts went diffused through pnc-Si membranes.  I was able to achieve this twice, although the second time took two days before the gold signal was intense enough to observe via…

Here are the results of diffusion based protein separations at different salt concentrations and 50kD Microcons: We see a sharp cutoff between 31 and 45kD.  Separations look like they have progressed further in 10mM salts rather than 100 mM (this…

The retrial on cellulose (100kD) gold separations gave similar results to the first study (results from Tecan scans): particle salt results 5 0mM no au in filtrate, retentate concentration 15 0mM no au in filtrate 5 10mM no au in…

This is just a little update on the results of the pnc-Si, cellulose, and pes separations.  When I added up the retentate and filtrate, it didn’t always equal the intensity of the starting sample (keeping in mind that I was…

Can viscosity be determined by dynamic light scattering?  Dynamic light scattering in essence measures the diffusion of particles and calculates the hydrodynamic radius using the Stokes-Einstein relation, D = kT/(6πηr).  To find a size using dynamic light scattering, you need…

We got a bunch of extremely nice EO wafers through the pipeline, and I’ve been trying to do some experiments with them.  There’s very few pinholes on these wafers, and the pores are beautiful.  My experiments so far have been…

I set up separations with 100kD cutoff Microcon (cellulose) membranes and protein standards.  Here are the results: Due to shorter than normal gel, the myosin didn’t get resolved.  However we can see a separation that appears to be fairly sharp. …

Here are the sizes of the particle ladder by TEM.  I compare to the previous unmodified DLS results in this table. Manufacturer’s Size TEM size Stdev DLS Size 5 6.6 .6 8 10 7.8 .9 9.4 15 12.7 .9 13.2…

This was the first try at using PES for protein separations by diffusion.  100kD cutoff Nanoseps were used to separate 10mM KCl and 100mM KCl standards.  In both cases, the backside fluid was gone after 24hours. I don’t think evaporation…

I reran the samples from this post on coomassie stained gels.  The sensitivity is lower, but the results look reasonable. First off I check out how well the sum of the retentate and filtrate matched the starting sample.  This was…