Below are the results of my recent crosslinking efforts. PEG with 1.5% crosslinker was exposed to UV light at varying times through a bar  printer/transparency mask measuring 100/200. In theory this corresponds to 100 micron PEG bars and 200 micron…

We have had success cross-linking PEG to TEOS membranes pretreated with TPM to aid in adhesion. This was done first on nonporous membranes, and then porous. We used 50% PEG at 1.5% cross-linker, which cross-linked at 8000ms. For comparison, 100%…

Working on the development of PEG microarrays for cell heterogeneity applications. Current work has been accomplished on TPM coated glass coverslips. Transfer to membranes are in progress. Three different x-linker concentrations were tested in a 1:1 mixture of PEG and…

A control was done to observe cell proliferation on a TPM coated cover glass. Images were taken at 10x after 4 days.  TPM coated cover glass  Polystyrene petri dish We are having some success with the seeding of bend.3 cells…

Updates: We can now put the glass on top of the mask, which allows the use of nonstick agents in the creation of PEG microarrays. The ability to cross-link PEG-Diacrylate (PEG-DA) with a photoinitator and UV exposure was previously tested and…