As I mentioned in the meeting this morning the SC267 samples have run out so I have begun testing SC269 for use in my experiments. This post pertains to a BSA diffusion experiment that I set up yesterday and allowed…
Author: Mike H
UV-Ozone treated SC267: DDM passes and BSA held back
Building on my recent posts I ran an experiment with SC267 testing the ability of BSA and DDM to diffuse through UV-Ozone treated (20min at 150C) and untreated samples. I used four samples testing both BSA and DDM in the…
Posted in NRG
Dye Diffuses through UV-Ozone Treated and Untreated SC267 Samples
In my last post I presented the results of an experiment in which it appeared that BSA did not diffuse through a SC267 sample treated in the UV-Ozone system for 20min at 150C. As a control I also ran BSA…
Posted in NRG
Diffusion of BSA through SC267 prevented using UV-Ozone
As mentioned in my previous few posts Justin and I have been working to characterize contact angles resultant from various exposures in the UV-Ozone system. Earlier this week we got our new oxygen tank installed and it appears that after…
Posted in NRG
UV-Ozone Contact Angle Measurements
Last week I made a post showing the diffusion of DDM and BSA through SC267. I an effort to increase the negative charge associated with the membrane material I proposed using UV-Ozone exposure. A while back there was some concern…
Posted in NRG
BSA diffusion across SC267
Last week I made a post showing detection of detergent diffusion across membrane SC267. In the diffusion set up I had placed 6uL of a 50/50 volume ratio solution of 1.0% DDM and 1mg/mL BSA with 60uL of DI-water on…
Posted in NRG
DDM diffusion through SC267
In a previous post I showed that a colorimetric assay using sulfuric acid and phenol can be used to establish a linear relationship between known detergent concentrations and absorbance values. This assay proves helpful in the project related to Mark…
Posted in NRG
DDM Colorimetric Analysis
After talking with Prahnesh, a graduate student in the Dumont Lab I obtained a protocol for a colorimetric assay used to detect the presence of detergents in solution. The assay uses phenol and concentrated sulfuric acid in the following manner:…
Posted in NRG
BSA-DDM Complex Intensity
I took a look back through the Melvern data I collected, particularly focusing on changes in the intensity data in the BSA-DDM solution (the previously posted plots were of number data). When I compared the peak intensities for BSA, DDM,…
Posted in NRG
BSA Size/Zeta-Potential and BSA-DDM Complex Results
Yesterday I used the Melvern to determine particle size and zeta-potential for BSA in DI-water. For the particle sizing I used a concentration of 2mg/mL but I had to increase the concentration to 10mg/mL in order to get an accurate…
