In preparation for Tuesdays meeting Jim asked me to prepare this chart to compare the results of the treated and untreated diffusion experiments from this summer. The chart values given are the percent concentration of the sample filtrate in comparison…

I made a post yesterday showing that in 20mM HEPES, pH7.5, +10mM NaCl solution equivalent amounts of DDM were diffusing through the “treated” and “untreated” SC256 samples. Additionally it appeared that minimal amounts of BSA were diffusing across either sample.…

Yesterday I posted the results from an experiment testing diffusion of BSA and DDM with salts (10mM) across “treated” and “untreated” SC256 samples. The results posted yesterday showed relatively equal amounts of DDM diffusion in both the “treated” and “untreated”…

In my last post I showed that using buffered solutions instead of Di-water we could obtained similar results with SC256 as we had with SC269. I used 20mM HEPES at pH7.5 with no salts and showed DDM diffusion through both…

On Friday I posted results from a diffusion experiment in which I tested “treated” and “untreated” SC256 samples against the diffusion of a mixture of DDM and BSA. The DDM-BSA solution contained an initial DDM concentration of 1.0% and an…

In an effort to conserve membrane samples I am working to run diffusion experiments with a mixture of BSA and DDM. This will only use 2 chips instead of 4. In our meeting on Tuesday it was mentioned that BSA…

After our meeting with Professor Dumont on Tuesday it was decided that we needed to try and perform the BSA/DDM seperation with a more porous membrane (to allow for eventual pressurization). SC256 was choosen and as an initial step I…

The last post I made about the BSA/DDM separations using “treated” and “untreated” SC269 samples explained a problem I was having in which the initial retentate drop was diffusing around the edge of the sample chip. It seems that SC269…

Yesterday I posted results of a diffusion experiment using rhodamine and fluoroscein that showed the pores in SC269 are open both before and after treatment in the UV-Ozone system. I set up another diffusion experiment to test “treated” and “untreated”…

Yesterday I posted my results from a BSA diffusion experiment using “treated” and “untreated” SC269 samples. To ensure that the pores in SC269 are indeed open I took a step backwards and set up a diffusion experiment using small dye…