With our virus sensor paper now published, my journey with virus diagnostics, at least for now, comes to an end. Keeping with the theme of modifying silicon surfaces for interaction with pathogens, I am now switching gears to developing models…
Author: Michael Klaczko
He gives his blood to science.
Seeing the Softer Side of Silicon: Polymer Coating Silicon Membranes Towards an in vitro Bacterial Infection Mimetic
Posted in NRG
Strutting the Runway: Modeling the Microfluidics and Reaction Kinetics of the μSiM-DX
The development of the μSiM-DX has been swift and focused, as discussed in these previous blog posts: https://trace-bmps.org/beadazzled-laying-the-foundation-of-the-%ce%bcsim-dx-fouling-based-sensor/ and https://trace-bmps.org/vindictive-virus-capture-testing-the-%ce%bcsim-dx-with-vaccinia-virus-towards-a-modular-virus-diagnostic/. To provide a greater understanding of the inner workings of the device, I have done a few calculations regarding some of…
Vindictive Virus Capture: Testing the μSiM-DX with Vaccinia Virus Towards a Modular Virus Diagnostic
After completing proof of concept testing with polystyrene beads and with the goal of creating a SARS-CoV-2 sensor (Discussed in this post: https://trace-bmps.org/beadazzled-laying-the-foundation-of-the-%ce%bcsim-dx-fouling-based-sensor/), we sought to test the performance of the system with a target more similar to the SARS-CoV-2…
Beadazzled: Laying the Foundation of the μSiM-DX Fouling-based Sensor
Starting in December of 2019, we began capturing fluorescent beads on microslit silicon nanomembranes as an illustrative representation of particle capture within our microfluidic devices. From then until now we have successfully used this method within gasket made TFAC devices…
Bad to the Bone: Hydroxyapatite Coating Silicon Nanomembranes
In support of the bone infection studies done in the Schwarz lab at URMC, I have recently been tasked with coordinating a new collaboration with Dr. Yates lab in the Chemical Engineering department. The goal of this collaboration is to…
Posted in NRG
SPIE Photonics West BiOS Conference Presentation: Rapid detection of intact SARS-CoV-2 viral particles using silicon nanomembranes
This year we were invited to submit a manuscript and presentation to the SPIE Photonics West BiOS Conference. We submitted both on the work we have done on the uSIM-DX virus sensor platform. Below I have attached the presentation which…
Posted in NRG
Unqualified Engineering: Modifying ALine Devices For Vaccinia Virus Capture
Since the arrival of ALine devices on the diagnostic scene during the SARS-CoV-2 sensor project, much of my effort has been focused on getting them to work properly with biological targets. With plenty of data showing successful operation of these…
Posted in NRG
Flow Through Affinity Based Capture or a Lack Thereof, the Story of a Chemist’s Folly
Fluorescent Streptavidin Coated Bead Capture on Biotin KODE Coated Surfaces After determining that KODE molecules can successfully coat our silicon pore membranes, we set out to determine if the coating could be functional and how stable it was. To test…
Posted in NRG
Cracking the KODE: Dialing in KODE Surface Coatings and the Beginnings of Chemical Capture
Since precision printing FITC KODE molecules into the windows of our 3 slot chips I have begun testing both the uniformity and the functionality of surface coatings of these molecules. In pursuit of coating uniformity I have tested several different…
Posted in NRG
Membrane Functionalization: The Demise of Polymer Chemistry and the Rise of KODE
Carrying our membrane functionalization work forward we attempted to work in both the polymer chemistry space and the KODE nonspecific binding space. With the results shown below the KODE molecule pathway has shown much more promise than the polymer chemistry…
Posted in NRG
