A quick recap of the goals and initial experiments: https://trace-bmps.org/electrospinning-aligned-polymeric-fibers-on-parylene-stensils/ After obtaining a reproducible optimized fiber deposition settings and an improved device design, we moved on to growing HUVECs on these devices as the next step. As previously stated, given…
Author: Shayan Gholizadeh
Electrospinning aligned polymeric fibers on parylene stencils: cell culture and pll-g-PEG coating update
Posted in NRG
Electrospinning aligned polymeric fibers on parylene stencils
The aim of this work was to electrospin aligned polymeric fibers on parylene stencils as means to characterize the critical necessary total area and necessary opening sizes to elicit endothelial cell response in the form of cell alignment among other…
Posted in NRG
Modification of parylene C/N membranes for altered cell-substrate interactions
The motivation of this work by Daniela Lincoln (master’s student) was to investigate the feasibility of using different variants of parylene (C and N) for barrier studies and to look deeper into the implications of plasma surface modification for cell-substrate…
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Exploring Formlabs SLA 3D printing for cell culture applications
The initial purpose of exploring formlabs SLA 3D printer for a collaboration with Dr. Nicola Marchi (link to previous post). The purpose of the this work was gaining the ability to use a simple membrane on 3D printed platform…
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Update on ECM alignment mechanosensing and cell polarization using microporous membranes as masks
As a quick update on the ECM masking with Parylene membrane, we made changes to the device designs. Instead of relying on the peel-off process, we used a simplified design, with the membrane serving as the roof of the channels.…
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ECM alignment mechanosensing and cell polarization using microporous membranes as masks
Objective: Determine the minimum required area and number of exploratory cytoplasmic protrusions for sensing ECM alignment and causing cell polarization. Hypothesis: Cells only need to sense collagen alignment through exploratory protrusions extended during initial phases of cell seeding and not…
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BMES 2019 Recap: Organ-on-a-chip models with emphasis on modeling blood-brain barrier
There were lots of interesting talks and posters on organ-on-a-chip models with emphasis on modeling blood-brain barrier. I have selected four of them as a summary. 3D model of diffuse intrinsic pontine glioma growth, migration and blood-brain barrier interactions Alanna…
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Modeling leukocyte transmigration thorough a blood-brain barrier model
This post is dedicated to our ongoing collaboration with Dr. Marchi at Centre national de la recherche scientifique (CNRS) in France. The basic concept of the project is simple (Fig.1) and involves attaching a microporous membrane on top a 3D…
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Tensile Mechanical Testing of Ultrathin membranes
As part of Aim 1 of my PhD, I am looking into mechanical characterization of ultrathin membranes to explore the effects of porosity on the structural integrity and determine how porous we can go with a porous membrane, and last…
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