First off, this is the protocol for the staining and analyzing the data is shown below and was used for all of the YAP experiments: For the substrates, we wanted to use 0.5μm HP, 0.5μm LP, 3μm HP, TCP, and nonporous…

To follow up on my recent posts about HUVEC YAP localization after 24 and 72 hours (https://trace-bmps.org/blog/data/2016/10/19/yaptaz-passage-one-staining and https://trace-bmps.org/blog/data/2016/10/11/yaptaz-staining-on-membranes-and-nonporous-sio2) , we conducted the same study using Adipose Derived Stem Cells.  Due to the fact that these are mesenchymal stem cells,…

After reading and discussing a paper in journal club that talked about cells having a “mechanical memory” (found here “Mechanical memory and dosing influence stem cell fate“), we decided to replicate our previous YAP/TAZ experiments with passage one HUVECs direct from…

To continue our spreading and proliferation experiments on various topographical features and stiffness, I repeated the HUVEC experiments I had done before due to a possible artificial bias that we think is a result of high seeding.  To enusre accuracy,…

To analyze the dimpled  Sylgard substrates that we’ve been making, we’ve been conducting experiments to investigate how topographical differences in the surface of a substrate impacts HUVECs and ADSCs, in this case the dimples.  We’ve already shown that cells will grow on these membranes in…