Just to give a brief background, I have been working with endothelial cells to make a microvessel mimetic device, and one of the important requirements for endothelial cell culture is to be able to grow the cells under physiological levels…

For last 1.5 years, I have been working on developing a COMSOL model to understand how the geometry of pncSi chips affects the TEER values when cells are growing on them. I addressed this issues in some of my previous…

I have been working on developing a microvessel mimetic using our silicon membranes. Just as a recap of vascular biology, the basic components of a microvessel are endothelial cells, the basement membrane secreted by them (presence of perciytes is a…

This paper parallels my efforts in creating a microfluidic device that can perform co-culturing of different cell types, allow real time phase and fluorescence imaging, and perform non-invasive TEER measurement using transparent electrodes. This is almost exactly what we aimed…

I am trying to grow endothelial cells under flow and measure electrical resistance of the culture during the flow-based growth. I am using a peristaltic pump to generate the flow. I am using microporous membrane with 3 micron pores with…

Name Place Email Description of Interests Status of Discussion Pierre Olivier, organizer Paris pierre-olivier.couraud@inserm.fr No contact Nicola Marchi (Luca’s friend) Montpellier, France Nicola.Marchi@igf.cnrs.fr  No contact Lester Drewes (Organizing committee) U Minn (organizing committee ldrewes@d.umn.edu  exosome islation from plasma  No contact…

This is the final post (hopefully) summarizing my simulation results I did to model Endohm system. Barrett used endohm cup system to do the coculture of bEnd3 and glial cells for his BBB studies. The resistance values obtained while using pncSi…

This blog is to revisit the old TEER data obtained by Barrett from the coculture experiments on pncSi and polymer membranes. The reason behind this analysis is to understand the differences of the raw resistance values obtained for the same…

I have been using ITO electrodes deposited on PTFE sheets for my TEER measurements. A 100 nm thin ITO is deposited on ~300 micron PTFE sheets, and have a sheet resistivity of 60 ohms/sq. This sheet is commercially available from…

I spent some more time in understanding the numbers I am getting for our device simulations. In my simulations, I am assigning a conductivity value for my cell layer. Technically, from this information and by knowing the thickness and area…