Paper can be found here I am working on developing an analytical model to understand how to accurately post-process the resistance values I obtain from my TEER measurements. This paper addresses a similar issue and comes up with a correction factor…
Author: Tejas Khire
Ph.D. candidate in Biomedical Engineering with a specialization in the development of vascular microphysiological systems (MPS) for studying disorders of innate and adaptive immune systems.
Variation of TEER values during cell growth in a transwell with chopstick electrodes
This is an addendum to the results I posted in my previous blog. I am trying to simulate cell growth on a transwell device and perform TEER measurements using a small chopstick electrode pair configuration. I modeled transwell membrane with…
Posted in NRG
Variation of TEER values during cell growth in microfluidic device
In my previous posts so far, I performed simulations to understand the behavior of TEER variation upon changes in electrode and membrane configuration. None of those models addresses the effects of cell growth on the TEER values. In real experiments,…
Posted in NRG
Variation of TEER with finite area membrane
In my previous post, I mentioned the effects of varying electrode geometry on the TEER value measured from COMSOL simulations. I had assumed that membrane has uniform porosity i.e. 100% active area, which is the case with the commercial polymer…
Posted in NRG
Variation of TEER as a function of membrane area (COMSOL simulations)
Background: This is a follow up to the blog that Jim wrote on the basics of TEER measurement. This is the link to my old blog, which also describes the theory and some other preliminary data about TEER measurements. The resistance (R) of…
Posted in NRG
Bonding PDMS to PET films
PET is a very common material used in various cell culture applications. Unfortunately it’s chemical inertness makes it immune to plasma activation, and we can’t bond PDMS devices to PET. I am working with a PET films coated with Indium…
Posted in Protocols
Variation of trans endothelial electrical resistance (TEER) with different membrane configurations
A bit of background: In order to quantify how permeable a tissue barrier is, we use a method known as trans epithelial electrical resistance (TEER). Cells are grown on a permeable growth substrate in a transwell format. When the cells…
Posted in NRG
Post-annealing of ITO deposited films
I am trying to create ITO electrodes for my microfluidic device for TEER measurement system. In my previous posts, I have shown that silver/silver chloride electrode, which is most commonly used in most commercial TEER systems, had killed the cells…
Posted in NRG
Cell growth in micovasculature mimetic device
I am trying to grow HUVECs under flow in my device to establish a tight and confluent monolayer for TEER analysis. My device schematic looks like this and the whole setup is as below I tried to use the NPN…
Posted in NRG Tagged with: cell culture, closed flow system, devices, microporous nitride, tissue barrier models
Cytotoxicity of silver chloride and platinum electrodes
I am working with different electrode materials as a substitute for ITO for my TEER measurement device. I used silver-silver chloride electrode and platinum-iridium ribbon (90:10) for that purpose. I made AgCl electrode by dipping about an inch of silver…
Posted in NRG
