It was decided during my rotation-review meeting to use a smaller PEG molecule (3-mer) for silanization of pncSi. Following is the brief description of the polymer. Boiling Point: 147-9°C @ 0.3mm Freezing…
Author: Tejas Khire
Ph.D. candidate in Biomedical Engineering with a specialization in the development of vascular microphysiological systems (MPS) for studying disorders of innate and adaptive immune systems.
Vapor based PEG deposition
This paper talks about PEG monolayer deposition using CVD. Here is the link for Ultrathin Poly(ethylene glycol) Monolayers Formed by Chemical Vapor Deposition on Silicon Substrates
Updates about PEG treatment
In collaboration with Alex Shestopalov’s lab, I started working on liquid based PEG treatment on windowed sepcon chips. The treatment is a 3-day long protocol and involves multiple washing and drying steps with different reagents. Most of our sepcon chips broke…
Posted in NRG
Hemocompatibility studies: Summary
Hemocompatiblity of pncSi membranes was tested by checking for three main parameters: 1) Platelet adhesion, aggregation and activation studies 2) Complement protein activation studies 3) Assaying levels of thrombin generation All the studies and their assays were based on the…
Posted in NRG
Hemocompatibility of pncSi – Complement Activation Studies
This is the third set of studies to assess the hemocompatibility of pncSi. It aims towards a quantitative analysis of the formation of the complement protein C3a in the blood upon interaction with pncSi surfaces. Biological Motivation: Complement proteins help…
Posted in NRG
Hemocompatibility of pncSi – Platelet activation studies
This is the first set of studies to assess the hemocompatibility of pncSi surfaces based on the paper by Fissell and Roy. It looks for the behavior of platelets in the plasma upon interaction with pncSi surfaces. Biological Motivation: Platelets…
Posted in NRG
Neutrophil activation on PEGylated glass surface
Hi All, This is my first blog on the NRG group. I am working on testing the biocompatibility and of PEGylated pncSi surfaces. To start with, I and Nakul began with checking the activation status of neutrophils on PEG treated…
Posted in NRG
