Author: Tejas Khire
Ph.D. candidate in Biomedical Engineering with a specialization in the development of vascular microphysiological systems (MPS) for studying disorders of innate and adaptive immune systems.

Bonding silicone gaskets with nanoporous nitride (Solved ?!?)

In order to resolve the silicon nitride- silicone gasket bonding issue, I, Josh, Tucker and Greg used different approaches to functionalize the nanoporous nitride (NPN) surface. Both surfaces of NPN, although have same material (silicon nitride), behave differently. The flat

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Development of microvasculature mimetic device

From last few months, I am working on the development of a co-culturing system that can work as an in vitro mimetic of the human vasculature. The idea is to make a device capable of co-culturing, real time imaging, equipped with

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Viability and growth of HUVECs on nanonitride membranes – Part 2

This is in continuation to the studies that I published here. I used two types of membrane surfaces: nano nitride trenches (the back side of membranes) with pure nitride on the surface, and zero-second-etch nano nitride with a completely intact

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Tuning Structure and Properties of Graded Triblock Terpolymer-Based Mesoporous and Hybrid Films

Tuning Structure and Properties of Graded Triblock Terpolymer-Based Mesoporous and Hybrid Films ABSTRACT: Despite considerable efforts toward fabricating ordered, water-permeable, mesoporous films from block copo- lymers, fine control over pore dimensions, structural character- istics, and mechanical behavior of graded structures

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Viability and growth of HUVECs on nanonitride membranes

So I got few nanonitride chips with a pncSi cap on it from Josh (#1019, 320 um sub, 50 nm SiN, 40/20). I annealed them before using it for cell culture. Instead of using the existing CytoVu designs, I made

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Changes in hydrophilicity of EA treated pncSi after storage in water

In my previous post, I mentioned that the EA treated chips showed decreasing contact angles after their storage in water in contrast to gel-pack or vacuum container. However the EA treatment was 4 days old, and I lacked adequate controls.

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Contact angles on EA treated pncSi

I etched 12 pncSi chips and gave it to Chris for EA treatment. I wanted to test 3 storage conditions: gel pack, vacuum container, and water, for total of 4 days including the day of coating as day zero. Unfortunately

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Proposed time-dependent PEG treatment studies

In my previous post talking about the contact angle measurement of 3-mer coated PEG, there was a lack of adequate controls, and also an unanswered issue of contamination of samples during storage. I would now like to refine the study

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3-mer PEG vapor deposition on pncSi: time stability studies

This is my first attempt of depositing PEG using vapor deposition methods, in a controlled fashion. The stepwise procedure: Josh was extremely kind by providing me with a diced pncSi wafer [windowless]. He taught me how to do the buffered

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Not exactly related..but a nice read

https://www.seas.harvard.edu/news-events/press-releases/harvard-scientists-design-new-adaptive-material-inspired-by-tears

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