Category: NRG

Permeability w/ PBS

In a first attempt to reduce the electrostatic drag that might be slowing water transport, I ran an experiment with 1x PBS.  The membranes were first ozone treated and then assembled into the SepCon inserts. From these first trials, it…

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Streaming potential -> Zeta potential

In this post I will describe recent streaming potential measurements for PES membranes and the calculated apparent zeta potentials from these measurements. Setup: 1. Fabricate Ag/AgCl electrodes: Silver wire at the anode in 1M KCl at 1mA current for 20…

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Water permeability with %50 EtOH soak, ozone

In an attempt to help wet the pores prior to permeability experiments, I’ve started soaking assembled SepCons in a %50 ethanol solution.  Each SepCon is inserted into an eppendorf so that the membrane side is fully submerged.  I realized that…

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Attempt at movable cutoffs

In the next paper that I’d like to publish, I will compare protein separations for two membranes with different pore distributions to a single membrane at two salt concentrations (basically a redo of this post).  My most recent trial with…

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Hydraulic permeability in the pressure cell

I’ve recently been exploring hydraulic permeability using the pressure cell.  As you may recall, the majority of our permeability measurements have been done in the centrifuge (EQ format or closed bucket).  The pressure cell allows for a more accurate measurement…

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DLS sizing for separations

I’ve updated by 3D separations figure by using actual DLS sizes rather than the dimensions roughly obtained from crystal structures.  I have run into a snag with reporting the data however – use sizes by intensity or sizes by number.…

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Looking at membrane impact with flux

In the previous post, I discussed how we could evaluate the flux of molecules through the membrane and alluded that this method could be used similarly to our effective diffusion coefficient manner of evaluating membrane impact.  Here are results of…

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Flux in membrane simulations

In this post I take a look at the flux of molecules through the 1-D membrane simulations.  I will then use this flux to compare to systems lacking a membrane. First off, how to find the flux.  Inside the membrane,…

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Reviewing membrane impact

To identify how much of an effect the membrane has on particular simulations, we originally came up with the idea of using a phenomenological effective diffusion to view the membrane impact (see this post).  Here is the most up to…

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Track Etched SEM

Because of the disagreement between the experimental and simulation comparison in this post, I decided to take a look at the Sterlitech membranes using SEM.  Barrett gave me a hand with all the images, and we tried to look at…

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