In my last diffusion experiment, I used wafer SC 442. The results seemed to show that IgG was indeed diffusing through the membrane. As a follow up experiment, I am trying to show that I can pass IgG through more…

As I was preparing for the BMES conference, I took a few pictures of endothelial cells on pnc-Si transwells that showed extensive vacuole/endosome expression (here).  The most dramatic example occurred on a 50nm-thick membrane that was barely porous.  So I…

The results of my last diffusion experiment regarding BSA and filtered IgG (using SC 364) were inconclusive. (See “Diffusion Test– filtered IgG and BSA”) Looking at the histogram of results, there is no clear difference between the absorption of filtered…

In my 1st post detailing glial cells on pnc-Si transwells, I saw lots of cell clumps above the membrane window (see here).  Since these cells were grown in the wells, I thought they could be piling on top of each…

Permeability was calculated with Dagan theory for membranes in this post.  Pore processing was performed and r.mat files were run through the programs used to calculated permeability in submitted Science paper. No Carbonization Experimental: 591 ul/(min*PSI*cm^2) Theoretical: 801 ul/(min*PSI*cm^2) Carbonized (6.2…

A simple hydraulic permeability study was performed to determine whether carbonization would negatively impact water transport through the membranes. 5 slit square SepCons from SC 471 were carbonized and put in the ultra-centrifuge with a 500 uL starting volume.  The…

SiMPore has been collaborating with the Infotonics Center to scale our SepCon production to a 6-inch wafer.  The wafers are patterned and deposited at their facility and then brought back to UR where we anneal and etch.  This week, we…

I’ve suspected that the barrier function of endothelial cells might be surface-dependent (that is, cells grown on the pnc-Si side vs. cells grown on the well side).  I hypothesized that the interface between membrane and well walls would alter the…

During the last production run, we experimented with varying the RTP annealing time (1 min vs. 5 min) at three different RTP temperatures (1050 C, 1100 C, 1150 C) in a 30 nm thick membrane.  Below are the TEM micrographs…