Permeability was calculated with Dagan theory for membranes in this post. Pore processing was performed and r.mat files were run through the programs used to calculated permeability in submitted Science paper. No Carbonization Experimental: 591 ul/(min*PSI*cm^2) Theoretical: 801 ul/(min*PSI*cm^2) Carbonized (6.2…
Category: NRG
Hydraulic permeability of carbonized membranes
A simple hydraulic permeability study was performed to determine whether carbonization would negatively impact water transport through the membranes. 5 slit square SepCons from SC 471 were carbonized and put in the ultra-centrifuge with a 500 uL starting volume. The…
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First attempts at SepCon on 6-inch wafers
SiMPore has been collaborating with the Infotonics Center to scale our SepCon production to a 6-inch wafer. The wafers are patterned and deposited at their facility and then brought back to UR where we anneal and etch. This week, we…
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Side-dependent barrier function
I’ve suspected that the barrier function of endothelial cells might be surface-dependent (that is, cells grown on the pnc-Si side vs. cells grown on the well side). I hypothesized that the interface between membrane and well walls would alter the…
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Zeta Potential Measurements for PES and Cellulose
Using streaming potential data collected via the flow device Jess created, I have attempted to recreate the Zydney findings for membrane zeta potential. Ag/AgCl electrodes were used in 10mM KCl, mimicking identically the method outlined in Zydney. Data are below:…
Pore morphology is RTP time dependent
During the last production run, we experimented with varying the RTP annealing time (1 min vs. 5 min) at three different RTP temperatures (1050 C, 1100 C, 1150 C) in a 30 nm thick membrane. Below are the TEM micrographs…
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Diffusion Test– filtered IgG and BSA
The human IgG did not seem to diffuse through the membrane the first time we conducted a diffusion experiment. Barrett believed that the lack of diffusion was most likely due to protein aggregates blocking the pores. So, the human IgG…
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Beginnings of a pore formation model
During the history of pnc-Si fabrication, we have always observed that pore formation is intimately related to the crystallization process. In general, large nanocrystals in a film correspond with larger pore diameters. A partially crystallized film will have a low…
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Monolayers, not clumps on pnc-Si membrane
In my last couple of posts with respect to endothelial cell culture on pnc-Si transwells, I presented images of cell clumps over the active pnc-Si membrane area of transwells (here and here). There were dead cells associated with these clumps…
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No edge effect with NG10815 cells
I’ve repeatedly seen cytotoxic edge effects when I grow bEnd3 cells on the bottom of PET membrane transwells. Here’s a post of images. I’m getting closer to co-cultures of bEnd3 and NG108-15 cells on opposite sides of membranes and I’ve…
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