The human IgG did not seem to diffuse through the membrane the first time we conducted a diffusion experiment. Barrett believed that the lack of diffusion was most likely due to protein aggregates blocking the pores. So, the human IgG…

During the history of pnc-Si fabrication, we have always observed that pore formation is intimately related to the crystallization process.  In general, large nanocrystals in a film correspond with larger pore diameters.  A partially crystallized film will have a low…

In my last couple of posts with respect to endothelial cell culture on pnc-Si transwells, I presented images of cell clumps over the active pnc-Si membrane area of transwells (here and here).  There were dead cells associated with these clumps…

I’ve repeatedly seen cytotoxic edge effects when I grow bEnd3 cells on the bottom of PET membrane transwells.  Here’s a post of images.  I’m getting closer to co-cultures of bEnd3 and NG108-15 cells on opposite sides of membranes and I’ve…

Last week the rotation stage broke in the middle of a deposition.  The wafer came out looking very non-uniform, and judging by the color of the film there was at least a 10 nm gradient.  Since I’m not sure when…

Last week, a RTP temperature series was performed on 15 nm Si membranes with 20 nm and 40 nm oxide layers.  The purpose of this study was to 1. evaluate the effect of a thicker oxide on pinhole density and…

There has been some confusion lately with what we should name the compartments in diffusion.  Here’s what I’ve come up with: Top/Compartment with initial sample: Retentate Bottom/Compartment to which things diffuse: Dialysate The original word we were using for dialysate,…

SepCon Experiment (Carrie): Does it work in a SepCon format? Use “Protein A Beads and IgG Binding and Filtering (with SepCon Format)” 9/29/09 Make Buffer B at pH 3 9/30/09 1) Rinse off ethanol 2) Add IgG Take absorbance reading…

Test of Binding/ Unbinding (Carrie and Barrett): Test to see if we can successfully bind Human IgG to the protein A beads and then remove it with buffer B. After ordering the new human IgG and protein A beads, I…

I  have attached (I hope) a paper one of my students and I wrote a long time ago on the hardness of porous silicon films vs. porosity etc. The references is Duttagupta,…, and Fauchet, Solid State Commun. vol 101, pp…