I reran the samples from this post on coomassie stained gels.  The sensitivity is lower, but the results look reasonable. First off I check out how well the sum of the retentate and filtrate matched the starting sample.  This was…

Dave did a series of 20nm SiO2 film depositions at different temperatures (constant bias) and gave them to me to AFM.  I guess he wanted to see if film roughness changed drastically over the range of ‘room temp – 400C’…

In this gel I evaluated the linear range of coomassie brilliant blue. From this test it looks like things are linear from .0156 to .125mg/mL.  My guess is that the protein ladder bands in the previous experiment fall within the…

Previously I have silver stained all gels because the separations look very clear with this staining technique.  However, the silver stain is not giving very quantifiable results.  Coomassie staining means a loss in sensitivity, but it’s the gold standard for…

I’ve always wondered what the variability was in the pore statistics extracted from TEM images of different fields on the same membrane (SC 141).  I took a series of ten images from random positions (corners, edges, center) and ran them…

After some experimentation with acetylene concentration and anneal temperature, I was able to find a carbonization recipe that appears to shrink our pores by 1/3 the original diameter.  Below are images of an untreated sample (TEM 148: 15 nm pnc-Si…

Before Anant left for India, we were doing cell adhesion studies with pnc-Si transwells and getting some weird morphologies.  Check it out here, here, here, here, here. This is in contrast with the 2D studies that Anant did for the…

First, last week I had some images taken with new “visiting” AFM. These were frontside of the membrane with parylene deposited from the well side and the front side with deposited parylene on it. 1-1. Parylene deposition was not successful…

Earlier this week, Maryna and I did some AFM of her samples.  She vapor-deposited parylene on the well-side of pnc-Si and was interested in whether/how well that polymer got through the pores.  We got some really interesting-looking scans, but I’ll…

I used the transwell track etched membranes for these permeability experiments.  I first looked at 5nm gold in 10mM KCl.  After 24 hours the retentate and filtrate had just about equilibrated: We can compare these scans to the stock 5nm…