Attached is a series of images we took of two populations of neutrophils above and below the membrane.I’m including by methodology for those who are interested:One population was pre-labeled with AF488 (green) antibody against L-selectin, the other with AF546 (red).…

A couple of summers ago a Penn State summer student named Joe Fantuzo was working in the lab measuring cell growth rates and cell spreading on pnc-Si membranes and comparing the results to glass coverslips and tissue culture grade plastic.…

20nm polystrene beads in 1x PBS passed through w218. This wafer looks a little strange, like it was not fully etched (Dave do you know what happened to this one?). This was just a first stab at the benchtop diffusion…

Earlier in the week I retested the 100K microcons in our wet-wet EQ format. When I last ran them this way I calculated very little flow and very small permeability values. I noted that there was a rather large air…

Today I looked at a wafer (391) that had been annealed in the RTP on top of a 6″ wafer substrate. The idea is that the six inch substrate would create a “shadow” around the 4″ wafer which would improve…

We’ve got pores! Unfortunately, we are still being plagued by an unknown contamination that seems to be “eating” away at our membranes. This would explain why we’ve been having a hard time separating. We also observe a strong radial dependence…

Our two O2 regulators arrived this week (it figures we would have two to arrive within a day of each other after waiting for weeks to have one). Tom and Lingyun got to work right away. The image below shows…

Coming to a basement near you by the end of 2008 (assuming no delays that is). Pretty exciting in my opinion. 

Samples are from wafers #316 and 318. This is the first time for wafer scale deposition. Processing conditions:1) chamber base pressure @ 6×10-7 Torr (previously @ 5×10-6 Torr)2) 15nm Ti + 15nm Au, no vacuum break Estimated “cell resistance”: about…

I few weeks ago I posted some flow rate data based on the 100K microcons in which three of the four membranes worked fine and the other appeared to be a “dud”. I pulled the four systems apart and used…