The new smaller EQ setup that Tom came up with were tested in these experiments. The benefit is that there is much less dilution of our filtrates. 500ul of our sample (in these cases we tested 1.0 mg/mL and 0.5…

First Try is detailed in this post. Three membranes from w334 were used to separate depleted serum. As before we see that all of the proteins pass through the membrane, and again we can observe the one middle band that…

click here for the powerpoint presentation click here for the PDF presentation

This gel shows the separation using two 100k Microcon membranes and 1mg/mL brain extract. 500uL of 1mg/mL brain extract was loaded into the Microcon and spun at 14000g for 5min. 400uL passed through the membrane (100ul remained on top). It…

Molar flow thought pores with different diameters under 1 psi. First, I calculated molar flow based on porosity histograms extracted from TEM images. And then compared it with the actual experimental data. Here are few examples of this. molar%20flow.ppt

Six wafers (385-390) were produced last week with the new Solaris 150 RTP system. Good news: We now have much better control/feedback compared to the RIT setup. There are two thermlecouples on the wafer tray that monitors the front and…

This is a side view schematic of the experiments I’m setting up in Mike and Derek’s stir cell. The membrane separates two wells. In the well where the membrane is flush with the wafer I add 300uL 5nm gold in…

The coating was done last week at RIT (it was postponed from two weeks ago because of the failure of the e-beam crystal sensor). I deposited 10nm Ti and 15nm Au, without vacuum breaking. All the samples are from wafer…

A droplet (3uL) of 0.117 uM actin solution was added on membrane W303(0,2). Let the membrane sit in humidity Chamber for 20 min. Remove excess liquid. Add equal volume of Uranyl Acetate on membrane. Wait 90 sec. Remove excess liquid.…

I am trying to build a helpful spreadsheet that outlines all the experiments perfomed with a particular wafer. It also lists the conditions and quality of the wafer. Any other suggestions would be helpful. To view the spreadsheet, click here.