Hi all – I finished my 10 months internship with Sartorius on 16th of July and since all my results are going to be the third and last aim of my Ph. D., I will be presenting my results in…

Hi all,   I have attached my presentation for 6 months PhD committee meeting explaining my internship project with Sartorius. Best Comittee Meeting

Hello Everyone, Here is a summary of my trip to Nashville to learn about extracellular vesicles in cancer and present my work on isolation of nanoparticles/EVs using ultrathin nanomembranes.  Main points of the conference:  This conference was about EVs in…

Hi everyone, One of the objectives in the last aim of my PhD is to investigate the effect of soluble factors secreted by adipose derived stem cells (ADSCs) on wound recovery of fibroblasts (FBs). We have categorized these soluble factors…

The goal of this set of experiments was to study the growth rate of cells in 3 different media; 1. Complete Media 2. Exosome Free Media 3. Serum Free Media. We studied the effect of media on both ADSCs and…

Introduction:  Fibroblasts: Dermal fibroblasts are cells within the dermis layer of skin which are responsible for generating connective tissue and allowing the skin to recover from injury. A fibroblast is a type of biological cell that synthesizes the extracellular matrix and collagen, produces the…

Hello Everyone, This is an update on all my experiments and analysis on PKH paper. As i had a post before about PKH nanoparticles and PKH-Exosome fusion after labelling, we’ve been working on analysis and finishing figures for this paper.…

Hello Everyone, So Shannon and i have been working on studying the effect of exosomes in different conditions for wound healing assays and random migrations. This post is about one of our experiments (wound healing) to give you an update…

Hello Everyone, I’ve been working on exosome labelling and trying to characterize it by NTA. So we read a paper published a month ago, explaining the presence of PKH nanoparticles as false positive results in internalization studies. They studied four…

Hello Everyone, The goal of these experiments was to study the effect of exosomes on wound healing. We wanted to study, first importance of exosomes  for migration of FBs and eventually closure (healing) of the wound and second effect of…