Hello Everyone, So Now that we have NanoSight in our lab, i decided to run some experiments with different conditions of media and compare the number of particles in each media. We designed this set of experiments like: Water DMEM…

Hello Everyone, So i decided to do some calculations on our 2 different sets of experiments (Track Etched and NPN) and compare the applied and the required force of them, in addition to making sure that we are applying enough…

Hello Everyone, As you probably know, i have been working on calibration curves for NPN and TE membranes and comparison of these two sets of experiments with each other. This post is all about how i made the calibration curves…

Figure 2 – Micro scale Experiments with 10 micron fluorescent particles and 8 micron pore size track etched membranes: A) Fluorescent intensity – time plot showing increasing in the intensity signal during the capturing step and decreasing for releasing step.…

Hello Everyone, For the next figure in our paper, and to make the transition happens smoothly from Track Etched membranes to NPN membranes, we decided to run the same beads experiment on NPN membranes as well. So Kilean and i…

Hello Everyone, Following of making figures for our first exosome paper, I needed to have SEM images after releasing step. So i ran another experiment and checked the membrane after releasing step. You can see the SEM images of this…

Hello Everyone, Following up the discussion that we had in my last post https://trace-bmps.org/sem-images-for-micro-nano-scale-experiments/about my SEM images after capturing and cleaning. It was suggested to tilt the samples and make sure that beads are inside the pores.    So I…

Hello Everyone, I have been trying to take SEM images in 3 different steps during my experiments with polystyrene beads over Track Etched membranes including before capturing, After capturing and releasing. Micro Scale Experiments:  As you might remember, in Micro-scale…

Hello Everyone, Please note that Figures are all shown in the powerpoint file. The goal of these experiments was to plot a standard curve for concentration and intensity in my Track Etched Membranes. In order to that, we decided to…

Hello Everyone, So following up on my exosome scale experiment and my last post, i am presenting my last update to summarize my results and to show that my results are trustable and reproducible. This is how we take images:…