Since we’ve been noticing slower discoloration in the Sepcon format, I looked at the 2 components of Sepcons – the plastic and the O-ring. Specifically, I cut pieces from w622 and incubated them in FBS-DMEM/F12 either alone, with the Sepcon…

XTT is a colorimetric assay for measuring cell viability.  Supposedly, it gets taken up by cells and is reduced by mitochondrial activity intracellularly into water-soluble orange formazan crystals.  The readout is the absorbance of these orange crystals. I used XTT…

My last cell post involved pictures of HUVECs after 1 day of growth on commercial transwells and pnc-Si Sepcons.  Over the past week, I’ve been growing HUVEC on commercial PC transwells and pnc-Si in the Sepcon format in order to…

I repeated the autoclave experiment from earlier this week, but this time I also used the Sepcon format.  I used wafer 662, which was contaminated with large spots.  This was done in the oven in RPMI-1640 with FBS (no RTP). …

My previous discoloration post showed that chips in the Sepcon format discolored much slower than flat chips.  A flat chip in the same well as a Sepcon chip seemed to accelerate discoloration.  One thing about the Sepcon format that I…

I’ve been doing some preliminary studies with HUVEC in commercial transwells and on pnc-Si in the Sepcon/transwell hybrid format.  The thinness of pnc-Si will enable better microscopic examination of cells than other membrane systems (if pnc-Si is flat).  I thought it would be…

I ordered some new AC160 tips last week from Asylum and got on the AFM Thursday to test them out.  I think they solved some of the problems I was running into over the last few weeks.  Mainly, I was finally able…

I performed a discoloration experiment with w639 in EGM + 10% FBS.  I put 2 blank chips in the Sepcons membrane side down.  I then placed one Sepcon in a well with EGM alone.  The other Sepcon was placed in…

This is a repeat of the previous array experiment, but with a higher NaHCO3 concentration (1.85 mg/mL). Also, since these experiments require a lot of samples, I decided to cut chips off of wafer skeletons.  This one was w622.  As…

Based on last week’s lab meeting, I made a “solution array” to test discoloration.  Specifically, I made 3 solutions of NaHCO3 (0, .23125, .4625, .925 mg/mL) and 3 solutions of NaCl (0.1, 0.5, 1 M).  I placed these non-RTP’ed w648…