Author: Barrett Nehilla

Discoloration – New wafers 2

Last week, I looked at discoloration with different wafer numbers (639,670,672,683,685,702,703), pre- and post-rebuild.  This experiment was done in the oven at 37C, in PBS and without RTP.   Based on the starting color, w670 and w672 are the thinnest

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Discoloration presentation

I’ve prepared a few slides of discoloration data for tomorrow.  The images didn’t copy over from the blog very well, but I think they are good enough for a discussion. labmeeting_oct211

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Discoloration – buffers

More discoloration experiments.  No wafers were RTP’ed, and I looked at different wafer numbers.  For each experiment, I looked at discoloration in the oven at 37C and the incubator.  The labelling might be a bit confusing.  For the top right

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Discoloration – oven vs. incubator

A couple weeks ago, I posted some pictures that showed a non-linear “dose-response” curve for NaHCO3 and discoloration in the incubator (post).  I repeated this experiment at 37C in the benchtop oven.   These pictures show that there was a

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New wafers – discoloration

Since Jess saw discoloration last week within a day in PBS with the new material, I did a couple of discoloration studies this week.  I looked at wafers 670 and 673 and compared them to 625, 704 and 680-T.  These

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AFM

I got back on the AFM today to rescan the wafers from yesterday and too scan a wafer that Dave had given me a few weeks ago.  The 2 panels are from the height channel, I used the 240 tip

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AFM retry

I posted some pictures yesterday from AFM last week.  I got back onto the AFM today and re-scanned the megasonics cleaned virginia semiconductor wafers.  For each image, the top and bottom panels are the height and ZSensor scans, respectively.  All

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AFM – Megasonics

I got some wafers from Chris last week(?) that had undergone different Megasonics cleaning treatments by the manufacturer.  We’re trying to figure out if these treatments are actually cleaning the wafers.  I’ve been having trouble getting the AFM to stay

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Tecan sensitivity

I did a quick experiment in order to show everyone what the Tecan allows us to do.  I made a dilution curve of rhodamine 6G in PBS and measured the absorbance and fluorescence with the fluorimeter. I first did an

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Sodium bicarbonate "dose-response"

We’ve been focusing on bicarbonate ions as one of the causes of discoloration. So, I made several sodium bicarbonate solutions and tracked discoloration of non-RTP’ed samples. I made 3.7 mg/mL NaHCO3 (=44mM, this is the concentration in DMEM), 1.85 mg/mL

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