To further investigate the mechanism of discoloration, I prepared buffers at different pH’s and measured discoloration and pH over time. Buffers were: 0.1M MES, pH=pKa=6.1; 0.1M Tris-Base, pH=pKa=8.39; 0.1M Tris-HCl, pH=pKa=8.35; 10mM HEPES in 1X HBSS, pH 7.38, where HEPES…
Author: Barrett Nehilla
AFM Training
Yesterday, Chris, Dave, Graham and I were (exhaustively) trained on the Asylum AFM in Rick Waugh’s lab. We had an overview of the basic features of the AFM and got some real data, as well. The top panel is a…
Posted in NRG
Time after RTP – Discoloration
Last week in lab meeting, someone wondered if there the time after RTP affects the rate of discoloration. To test this, I RTP’ed chips from w638 in Ar at 800C for 5 minutes. I then followed discoloration in DMEM+10%FBS in…
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Conditioned DMEM – re-try
Last week, I did a couple experiments based on a suggestion by Tom. The pH of DMEM starts out quite high and then buffers down into physiologic range after some time in the incubator. Tom surmised that a short exposure…
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Conditioned DMEM – Discoloration
Motivated by Tom’s suggestion at lab meeting on Tuesday, I tried “conditioning” the DMEM+10%FBS before adding the chips. “Conditioning” just means that I allowed the DMEM+10%FBS to equilibrate in the incubator for an hour before adding the chips to test…
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SiO2-deposited discoloration
Last week, Lingyun and I deposited 30nm SiO2 onto both sides of wafers 335, 341 and 345. Unfortunately, these wafers weren’t in too good shape, so there are only a few intact slits without pinholes. I then took a few…
Rhodamine transport in transwells
I finished the first experiment to look at rhodamine transport across commercial transwell membranes. I was mainly interested if there would be enough transport into the basolateral chamber to measure the rhodamine with the spec. Experimental details: both chambers in…
Discoloration – Cell Media
I’ve been learning the discoloration protocol from Karl and Anant and finally have some pictures of my own to show. We got a couple of new media (RPMI and DMEM/F12) last week in order to grow the cells I’m getting…
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Sepcon-Transwell hybrid devices
I’ve been working with the Corning transwell inserts and our SepCon units a bit recently. The addition of the Dremel last week has allowed better control while cutting the transwells, even without the right angle attachment (which will allow cutting…
