I have had a chance to play with some of the chips from Lot 1278, specifically the design with the 2 micron slots. Results are a bit of a mixed bag. Wetting these chips continues to be a challenge. My…

I’ve now made 18 pores in monolith devices, with only 1 real success, which was the first one that I report on already. All of the rest have failed for various reasons, mainly relating to the silicon nitride membrane in…

I have three monolith experiments to report, one of which was successful. In all three cases, I was able to make a pore, though it took about 5 times longer than it would have for the NPN-tented chips. This is…

Just a quick update on my status: I got the NPN pre-filters from Greg in Montreal and finished testing 15 of them last week, so this week I will start doing additional experiments. I plan to explore the size separation…

First off, a quick update on the state of the lab. The day finally came, we had to pack up and put everything in storage, so unless there is a very pressing need, I’m done data collection for the time…

Since my last update I have been trying to achieve reliable size-separation of DNA by length using dwell time with the NPN pre-filter. Originally I had hoped to achieve 250-bp resolution near the optimal length for the NPN filter devices…

This update covers my efforts since our last skype meeting to show DNA size separation in a filter, as well as some very interesting TEM images of ALD-coated NPN and associated controls. Here is an inventory of my current chips:…

Just a quick update this time – since my last post I have been doing control experiments to demonstrate the effect of the filters. For the controls I have been using the chips without the nitride cap, since I am…

Since my last update things have been going quite smoothly. Most pores I make are able to pass 3 or 4 samples before clogging, and I have filled in the various graphs quite nicely. I still would like to flesh…