MPS-2024-KC-1
Author: Chloe Chen
MPS 2024 slides-Investigating Impact of a “Cytokine Storm” on Astrogliosis in Human Blood-Brain Barrier in a Microfluidic Model of Sepsis
Posted in NRG
Exploring primary human microvascular endothelial cells for μSiM-hBBB modeling
Introduction Sepsis is a maladaptive response to infection with a 25-50% mortality rate and a high incidence of cognitive decline among sepsis survivors.1-6 Because infection originates outside the brain, the factors responsible for cognitive decline in sepsis must get to…
Posted in NRG
Investigating the potential of astrocytes as a neuroinflammation markers by assessing their activation state
Introduction Astrocytes, which are the most abundant glial cells and key components of the BBB, are known to play crucial roles in initiating and responding to neuroinflammation.1 Astrocytes’ activation after injuries suggests that they could potentially serve as a marker…
Posted in NRG
Culture divider insert assembly and test
Introduction We are designing a cell culture chamber insert to allow side-by-side co-culture of cells and ultimately triculture in the µSiM devices. Materials and Methods ALine devices assembly according to the “ALine Modular Device Assembly” instructions. 2-slot NPN membranes applied…
Competitive ELISA assay to measure the HA shedding from HUVEC monolayer
Competitive ELISA assay to measure the HA shedding from HUVEC monolayer Introduction The endothelial glycocalyx is a dense layer of proteoglycans, glycoproteins, and glycosaminoglycans (GAGs) that helps maintain the integrity of the endothelial cell (EC) barrier.1 The main GAGs are…
