This past week I ran multiple experiments using the pressure cell and sep-cons. I used membranes from W684 for the sep-cons since the burst pressure of these memebranes is high (around 3.65-10Psi). I added 100uL BSA to the Sep-Con and…
Author: Rachel Twardowski
PDMS, diffusion and pressurization update
I’m having trouble getting the PDMS to cure on the NC. When I first started these experiments in August, the PDMS seemed to cure on the NC without a problem. Now, when I remove the PDMS + NC from the…
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BSA detection on NC
Creating an array to hold a pnc-Si wafer to proving to be difficult. The PDMS spreads too rapidly though the NC to form a consistent array for the pnc-Si membrane (and eventually wafer) to rest on. I’m still running different…
Update on NC detection method
What has been accomplished thus far: Able to detect BSA on NC membrane with the use of Ponceau S stain With PDMS array on NC, no BSA leaks through the area covered by PDMS Initial trial done with 10:1 ratio…
Various methods of applying PDMS to NC
During the past week I tried various methods of applying PDMS to nitrocellulose. Below is a table of what the PDMS array looks like on the NC before curing in the over, and after applying 1mg/mL BSA and staining with…
Diffusion on shaker
I ran a few experiments using the stir cell on the shaker table. Diffusion occurs faster on the shaker, but more air bubbles form around the membrane, thus preventing further passage of rhodamine. The first experiment I ran was with…
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PDMS array on nitrocellulose
Below is an image of the PDMS array on the nitrocellulose membrane. I’m trying to create a stamp that can be applied to the NC membrane, before PDMS is poured on. The idea is that the stamp will be heavy…
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Staining nitrocellulose and PDMS array
I’m trying to find the best method to stain proteins on nitrocellulose, so we can easily see the protein on the NC membrane. I first tried Coomassie Blue which did not work very well with the NC. The Coomassie was…
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Diffusion tests with Nylon and Nitrocellulose
Last week I tried to diffuse Cytochrome C through our membranes onto PVDF and PES membranes to see if we can detect protein adsorption on the other membranes. The trial illustrated that cytochrome c will diffuse through our membranes and…
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Smith Lab Diffusion Studies
Harold Smith at the Med Center proposed an experiment for Jess and I to try, in which we use our membranes to separate proteins onto a PES, PVDF (Polyvinylidene fluoride) or Nitrocellulose membrane, similar to a Western Blot of DNA…
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