I mixed made solutions of PBS and Rhodamine with varying Rhodamine concentrations and ran them through the spectrometer. Below is the concentration vs absorption curve I made from it. I blanked the spec with PBS instead of ddH2O since the…

Art is going to make a second stir cell this week. I asked him to replicate it as closely as possible, however I’m not sure how well that’s going to work. The one we currently have is made out of…

I ran another diffusion cell last week using Human Albumin and Cytochrome C. I increased the volume of PBS because the last time I ran the gel for this mixture, the band for cytochrome c was very prominent. Out of…

I re-ran the rhodamine dilutions narrowing in on the molarities within the range readable by the spectrometer. My start sample was .5mM of Rhodamine. The wavelength I set the spectrometer at was 530nm with 3 trials of each dilution. I…

I fixed the graph for the serial dilution of Rhodamine that I did last week and removed all absorbency values that were greater than 1.08 AU. The graph looks much more linear now with an R^2 value of .9517

I ran a series of serial dilutions with the rhodamine, to find the absorbency using the spectrometer. Below is a graph of the concentration of rhodamine versus the absorbency. I removed the concentrations which had absorbances greater than 1.7 AU…

Last week Jess helped me set up the diffusion cell with human albumin and cytochrome c. We ran 3 cells and used membranes from wafer 565. The membranes used were -1,4 (retentate 1), 3,3 (retentate 2), and 4,2 (retentate 3).…