Introduction As we’ve been trying for nanoparticle-based CAD-IA for multiple trials, we have tested various reaction routes, reaction conditions, and antibody pairs with 3 different nanoparticles. Here, I would like to take a step back to go through the CAD-IA…
Introduction As what we’ve learned from the previous “Catch-and-Display Immunoassay” (CAD-IA) trials, we switched to an antibody pair from OriGene with low non-specific binding ability to each other and start to conduct ultracentrifugation for all reactive reagents for the reduction…
Introduction The glial fibrillary acidic protein (GFAP) is an intermediate protein released by astrocytes and has value as a serum-based biomarker of neurological abnormalities including traumatic brain injury1. Inspired by the CAD-LB method for digital assessment of biomarkers on small…
