I recommend we review this article in NRG or journal club – it was just published online in Advanced Functional Materials this week. I see a lot things in this paper that appear to be motivated by our work. They showed SFM/AFM…
Author: Thomas Gaborski
Burst Pressure Tests of Annealed and Unannealed SiO2 Membranes
Overview I burst pressure tested TEM chips with 1×1 mm windows of 100 nm SiO2 that were both annealed and unannealed (provided by JP and Josh Miller). In our experience it seems that the forming gas (H2/N2) annealed chips…
Posted in NRG
Adipose Derived Stem Cell Differentiation – Recap
Over the weekend, I resubmitted our adult Adipose-Derived Stem Cell (ADSC) paper titled: Membranes Promote Endothelial Differentiation of Adipose-Derived Stem Cells and Perivascular Interactions There were three main findings: 1.) ADSCs can be induced to express CD31 (PECAM-1) like endothelial…
Posted in NRG
Mechanical memory and dosing influence stem cell fate
This is a recent paper in Nature Materials that shows stem cells are influenced by prior culture on varying stiffness substrates. This means that when cells are induced towards adipogenic or osteogenic lineages, their prior culture history on a stiff or soft…
Posted in NRG
Creating Endothelial Cells from Adipose-Derived Stem Cells
In conducting experiments to address reviewer comments, we again created endothelial-like cells from adipose derived stem cells (ADSCs). As in the past, the vast majority of ADSCs express some CD31 (PECAM-1) after culture in endothelial media (M200) supplemented with 50ng/mL…
HUVEC spreading on various microporous SiO2 membranes
This is part of our work studying how cells adhere, spread and proliferate on varying pore size/porosity SiO2 membranes. This post will be a qualitative look at how HUVEC spread over membranes by staining for actin filaments. In a future…
NanoDays 3/29 & 3/30
We still need help with NanoDays http://doodle.com/paq66krxtf7mt6tk There are two time slots: 10:45am-2pm 1:45-4pm Please wear your I Love Nano shirts from last year. I have a couple more size Medium and one size Small. The Rochester Museum & Science…
Posted in NRG
Surface modification of PDMS for enhanced cell adhesion and proliferation
PLEASE read this journal article for NRG on Tuesday March 18th @ RIT (PDF links are at the bottom) Surface Chemical Modification of Poly(dimethylsiloxane) for the Enhanced Adhesion and Proliferation of Mesenchymal Stem Cells Shreyas Kuddannaya,†,§ Yon Jin Chuah,‡,§ Min Hui…
Posted in NRG
ADSC Endothelial Differentiation Manuscript
Membranes Promote Endothelial Differentiation of Adipose-Derived Stem Cells and Perivascular Interactions Submitted to Journal of Cellular and Molecular Bioengineering (a BMES journal) ADSC-All-Figures FIGURE 1. Ultrathin microporous silicon dioxide (SiO2) membranes. (A) 300 nm thick SiO2 membranes were fabricated…
Posted in NRG
Adipose Stem Cells on Nitride LiftOff Membranes (Part 2)
Background: In a prior post I showed that we had seeded cells on both nano- and micro-porous SiN Liftoff Membranes. The nanoporous proved to be rather weak and showed numerous broken windows after seeding the cells. Summary/Conclusions: In this follow up post,…
Posted in NRG
