We have successfully plated adipose derived stem cells (ADSC) on microporous silicon nitride (SiN) membranes. Cells plated on nanoporous SiN appeared fine at time of seeding, but membranes were broken at 2 hours – possibly due to media washing or…
Author: Thomas Gaborski
Microporous Silicon Dioxide (TEOS) vs. Nitride Imaging
After a false start with non-microporous TEOS chips, I quickly did an image comparison with truly porous chips using polystyrene beads. The TEOS and Silicon Nitride chips were both fabricated in the single-well CytoVu format (single 2 mm window, 5.4mm…
Simple PEG crosslinking on a microscope
We have re-started the PEG x-linking project at RIT. One of the goals is to create microwells made of PEG on top of a membrane in order to culture individual cells or small colonies of cells with the potential for…
Posted in NRG
EO Pumps using Track Etched Membranes
A group published a paper in Lab on a Chip (2012) using track-etched membranes for electro-osmosis. There are a lot of eerily familiar terms and concepts in the paper. The title even sounds familiar: Low-voltage electroosmotic pumps fabricated from track-etched…
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ALD strength on 5.03
To follow up on some of Dave’s work – I was curious if the ALD alumina affects the strength of pnc-Si membranes. Punchline: 2.5 nm of Alumina seems to increase the burst pressure, while 5.0 nm reduces the burst pressure.…
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Pressurized Gold Separations – Part 3
Dan Mendelson performed gold separations using wafer 255 and has shown that this wafer has a cutoff for gold near 25 nm. Below I have plotted the data of filtrate gold concentration as a function of the feed concentration for…
Pressurized Gold Separations – Part 2
I continued work with a couple of new wafers and settled on one that lowered the cut-off to between 5 and 10 nm gold particles. For completeness, I will also include some of the old data that has been further…
Posted in NRG
Pressurized Gold Separations
Motiviation and Background: Nakul and I have been performing a lot of DNA filtration and attempted DNA shearing experiments. We found that large DNA fragments (>1000 and >2000 bp) pass through ~30-40 nm max pore size membranes when pressurized to…
UV-ozone follow up
So I think I have confirmed that PDMS is at least one of if not the major contributor to the contact angle problems in the UV-ozone system. Control image before any treatment: I then dried the chip and placed it…
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UV-Ozone was broken, now fixed?
Nakul and I noted last week that the UV-ozone system was not performing. We took pictures of control and treated samples (2x) to measure contact angles on Amorphous, pnc-Si, Oxide and Nitride TEM grids. Our results were pathetic – most…
Posted in NRG
