This post presents the data from all of my TEER experiments of BBB co-cultures, with bEnd3 endothelial cells on the bottom and NG101815 glia on the top of PET and pnc-Si transwells.  For all experiments, I used 50000 cells/cm2 as…

A few weeks ago, I posted some high resolution (100X) images of BBB co-cultures stained with 3 different colors.  I also included the z-height of the different focal planes of cells on either side of PET transwells.  In this post,…

To follow up my last post on air-blood barrier TEER results, I wanted to show some Live/Dead images of these samples.  These are stained after the 14 day experiment. In the following image, you can see normal bEnd morphology on…

I finished 3 independent experiments of air-blood barrier co-cultures – these consist of bEnd3 endothelial cells on 1 side and RLE-6TN lung epithelial cells on the opposite side of the membranes.  We didn’t expect these cell types to potentiate TEER…

As a continuation of the substrate bias series, I deposited a 15 nm film with 05 W substrate bias and annealed at 1000 C. TEM shows that this condition generates a 13.4% porosity and a max pore size of ~40…

Last week, I reported on the the morphology of pnc-Si deposited with different substrate biases. We hypothesized that the reason for this was due to the different initial film stress of the as-deposited a-Si. Unfortunately, the pressure-displacement method does not…

Hi everyone! I’m sifting through the mass of data I collected during my last month and am compiling posts to update everyone. This post shows the latest with the microfluidic chamber that Henry and I designed.  In my first post…

It seems that pores are only formed in the “sandwich structure” on certain process. So in order to verify this conclusion, I annealed two layers structure (20nm sio2+15nm si) to test the agglomeration theory. The other parameters in depositing and…

UPDATE: A look at long and short crystal dimensions (9/1/10): DLS vs. Crystal Sizes – Newest version 8/30/10 Here is a comparison of sieving curves using DLS or estimated crystal dimensions of proteins. DLS: 24 hours sieving curve. 48 hours…

We have always speculated that the substrate bias during the a-Si deposition had a strong effect on pore morphology of pnc-Si. Here, I looked at three 15 nm silicon films deposited with 00W, 25W, and 50W substrate bias. Recall, that…