I first wrote about quantifying the pore formation process using kinetic theory last year. In this post, I will develop my thoughts a bit more. In the literature, researchers oftentimes present charts, like the two shown below, describing the Arrhenius…

I reported last week that a 15 nm silicon film deposited with 05 W substrate bias yielded a highly porous film. This week, I wanted to confirm this effect as well as compare it to films deposited at 10 W…

These experiments were conducted during the week of 5/10/2010 – 5/14/2010. To recap on this project, Barrett and I have been working with Dr. Harold Smith in the hopes of developing a drug screening test that consists of small-scale diffusions.…

In my last post, I presented the results of my BBB TEER experiments.  For the BBB co-culture, there was a spike in TEER.  I hypothesized that this was caused by media acidification by rapidly proliferating glial cells, which in turn…

This post presents the data from all of my TEER experiments of BBB co-cultures, with bEnd3 endothelial cells on the bottom and NG101815 glia on the top of PET and pnc-Si transwells.  For all experiments, I used 50000 cells/cm2 as…

A few weeks ago, I posted some high resolution (100X) images of BBB co-cultures stained with 3 different colors.  I also included the z-height of the different focal planes of cells on either side of PET transwells.  In this post,…

To follow up my last post on air-blood barrier TEER results, I wanted to show some Live/Dead images of these samples.  These are stained after the 14 day experiment. In the following image, you can see normal bEnd morphology on…

I finished 3 independent experiments of air-blood barrier co-cultures – these consist of bEnd3 endothelial cells on 1 side and RLE-6TN lung epithelial cells on the opposite side of the membranes.  We didn’t expect these cell types to potentiate TEER…

As a continuation of the substrate bias series, I deposited a 15 nm film with 05 W substrate bias and annealed at 1000 C. TEM shows that this condition generates a 13.4% porosity and a max pore size of ~40…

Last week, I reported on the the morphology of pnc-Si deposited with different substrate biases. We hypothesized that the reason for this was due to the different initial film stress of the as-deposited a-Si. Unfortunately, the pressure-displacement method does not…