Hi everyone! I’m sifting through the mass of data I collected during my last month and am compiling posts to update everyone. This post shows the latest with the microfluidic chamber that Henry and I designed.  In my first post…

It seems that pores are only formed in the “sandwich structure” on certain process. So in order to verify this conclusion, I annealed two layers structure (20nm sio2+15nm si) to test the agglomeration theory. The other parameters in depositing and…

UPDATE: A look at long and short crystal dimensions (9/1/10): DLS vs. Crystal Sizes – Newest version 8/30/10 Here is a comparison of sieving curves using DLS or estimated crystal dimensions of proteins. DLS: 24 hours sieving curve. 48 hours…

We have always speculated that the substrate bias during the a-Si deposition had a strong effect on pore morphology of pnc-Si. Here, I looked at three 15 nm silicon films deposited with 00W, 25W, and 50W substrate bias. Recall, that…

These are cross sections of my comsol diffusion model.  Each chip has 6 square wells terminated in a membrane.  This cross section cuts across the two middle wells.  Concentration contours are in 0.025 intervals. 6 hours: 12 hours: 18 hours:…

Over the last couple of weeks, I’ve been doing confocal fluorescence microscopy over at the Med Ctr.  I was hoping this tool would clean up some of the background fluorescence in my immunofluorescent images.  More importantly, the confocal can take…

As a proof-of-principle, Henry and I have been working on a microfluidic device for flowing blood cells/particles across cultured cells on pnc-Si.  More on why this is important later.  We’ve come up with a prototype and I’ve been testing it…

One of the points I’d like to discuss in my co-culture paper is the advantageous imaging qualities of pnc-Si compared to ‘translucent’ commercial transwell membranes.  This has been evident in many of my posts of Live/Dead stained cells, even at…

In order to figure out the relationship between grain size and pore size, I tried to use XRD measurement to determine the grain size by Scherrer equation. t = kλ/(B cosθ) where t is the average grain size, k is…

I’ve been trying to get multi-color images of endothelial cells and glial cells on transwell membranes in order to compare image quality on glass, pnc-Si and polymer membranes.  Specifically, I’ve tried staining endothelial cells green with VEGF mAbs and glial…