I had some extra samples from last week and had been wanting to try SEM on cells, so I went for it this week. Specifically, I was hoping to get images of vacuoles in cells over free-standing pnc-Si. I followed…
Category: NRG
Amyloid-Beta separations
I’ve been working recently with amyloid-beta (AB) provided to me by some people in Brad Nilsson’s lab. These experiments are related to the HMM diffusion experiments for our collaboration with Harold Smith. Basically, AB fibrils are very long (microns) and…
Posted in NRG
Incorporating Debye Length into EO Theory
During my previous derivations of electroosmotic theory, I described the electroosmotic velocity using the classical Helmholtz-Smoluchowski equation: where ε is the permittivity, E the electric field, ζ the zeta potential, and η the viscosity. This equations basically says the velocity…
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More vacuoles on free-standing than supported pnc-Si (and PET)
One of the other suggestions from Angela Glading at lab meeting a while ago was to quantify the vacuoles on different culture areas. So, I selected images from 3 different experiments (all at day14 of growth) and counted the number…
Posted in NRG
HMM vs. LMM diffusion
My last post dealing with this project was the first to definitively demonstrate HMM diffusion through pnc-Si. Unfortunately, there wasn’t a difference between the HMM filtrate signal for intact and digested (+RNAse) samples. As a reminder, HMM is a large…
Deflection of perforated silicon nitride membrane
In order to verify our measurement technique for the determination of Young’s modulus of pnc-Si, I decided to look at a PECVD silicon nitride membrane SiMPore is using for cell culture applications. The membrane window is 1 mm x 1…
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Membrane deflection revisited
In my initial report on the Young’s modulus of pnc-Si, I used the generalized pressure-displacement equation to fit my experimental data to extract a Young’s modulus of around 100 GPa (Polysilicon is ~150 GPa). Unfortunately, there was quite a bit…
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Epithelium on transwells(2)
My last post was the first experiment I performed with rat lung epithelial cells on transwells. It seemed like there was vacuolization but it was delayed compared to endothelial cells (and only happened on 1 of 2 pnc-Si transwells). Here,…
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Lung epithelial cells on transwells(1)
A couple weeks ago when I presented at lab meeting, Angela Glading suggested that I look at epithelial cell vacuolization on transwells. This was in order to determine if vacuole formation was endothelial cell-specific. If I’m remembering correctly, the rationale…
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HMM Diffusion through pnc-Si Sepcons
Carrie and I have been trying to detect HMM (APOBEC3G) diffusion through Sepcons without much luck. We determined that the dilution factor (~1:30) for diffusion in our 384-well plate format was too high to detect HMM in the basolateral well. …
Posted in NRG
