A couple weeks ago, I presented a potential figure for my paper that didn’t make sense.  So, I did a couple more controls and put together this new figure.  This is the “diffusion through ‘non-porous’ SC348/cut-off of nonporous membranes” figure. …

In the soon to be submitted paper on diffusion models, we list two contributions to membrane resistance.  The first is due to the steric and frictional hindrance a molecule encounters as it enters and diffuses through a pore (Renkin, Deen).…

This post is related to the HIV drug screening project that Carrie has been working on.  To refresh everyone’s memory, the aim of this project is to use pnc-Si as a filter to separate high molecular mass (HMM) protein-RNA complexes…

I first reported the crystallization of free-standing amorphous silicon membranes in this post in January. The last experiment was done inside the susceptor (top and bottom piece). This week, I annealed a set of amorphous 15 nm and 30 nm…

As a follow up to my 15 nm post-Si deposition Ar sputter experiment, I fabricated a set of six wafers with 30 nm Si films this week: three with the Ar sputter process and three without. I annealed these at…

The mechanism of organic dye photobleaching is not well understood, but there are a few methods out there for reducing the rate of photobleaching.  Some literature suggests that photobleaching is due to excess oxygen in solution and the interaction between…

This is simply the latest repeat of a blood-brain barrier co-culture on PET and pnc-Si transwells.  These are P17 bEnd and P24 NG10815, each seeded at 50000 cells/cm2.  As usual, I seeded bEnd on the pnc-Si side, upside down, and…

In most of the images I’ve posted of bEnd3 on pnc-Si, it seemed like there were more cells crowded onto the free-standing pnc-Si compared to supported pnc-Si.  This post shows that this is indeed true. To do cell counts, I…

All of my recent posts on VEGF, VEGFR2 and NF-H imunofluorescence have included data from cells grown on coverslips only.  This was my first attempt to try to do immunofluorescence staining on intact pnc-Si transwells and then pop the chips…

Recently, I have been exploring the temperature dependence of post-Si deposition Ar sputtered films (15 nm thick). Below is a comparison of the average pore size, porosity, and density of pores of Ar and non-Ar sputtered films annealed between 800…