In order to validate the salt diffusion test I just posted, I’ve been measuring H2O2 diffusion with SC348 and the Amplex Red Assay from Invitrogen.  I first showed that H2O2 (=34 Da) passed SC348 in this experiment.  Here, I just…

About a month ago, I posted some preliminary data that showed NaCl diffusion through SC348.  This was motivated by my observation of vacuole formation on SC348.  Since my hypothesis is that cell culture substrate permeability is the key factor for…

For my BBB project, I’ve wanted to get nice images of glial cells and endothelial cells on opposite sides of pnc-Si membranes to demonstrate 1) optical transparency 2) that both cell types are in focus (and thus right on top…

Membrane chips were amino-silanized with APTES in the YES system.  I performed the standard electroosmosis tests with these chips.  Here are the flow rates in comparison with non treated and oxidized membranes: Here are the calculated zeta potentials: As you…

A simple experiment that has never been done is to make a direct comparison between annealing inside and outside the susceptor. Intuitively, we would expect the morphologies to be quite different since the heating mechanism is different for both cases.…

I  carried out experiments to test the adsorption of 1 mg/ml of BSA on NHS-PEG surfaces.This time the APTES layer was deposited from solution phase on to the silicon surface in contrast to vapor phase (YES) deposition. Again adsorption of BSA…

I few weeks ago, I had a couple wafers made with different size squares and slits.  I posted my first results here and here.  In that experiment, I showed pretty dramatic 2D structures over both supported and free-standing membranes with…

Today I deposited two 30 nm thick Si films and annealed one at 1000 C for 1 min and the other at 1000 C for 5 min. My running hypothesis is that performing an Ar sputter after the silicon deposition…

In a continuing study on how a post-silicon deposition Ar sputter affects morphology, I annealed three 15-nm films for 10 s, 60 s, and 300 s. If you recall in my first report, I found that the Ar sputter increases…

I tried to measure the thickness of the non-carbonized and carbonized membranes for the paper using SEM. To have a clear cut of the membrane I place the membrane chip upside down on a silicon wafer piece, then bend the…